番茄汁对人前列腺癌PC-3细胞增殖和凋亡的影响  

Effects of Tomato Juice on Proliferation and Apoptosis of Human Prostate Carcinoma PC-3 Cells

作　　者：罗琼[1] 庞雅琴[1] 崔晓燕[1] 王丽红[1] 闫俊[1] 杨明亮 曾秀林 

机构地区：[1]武汉大学公共卫生学院卫生学教研室,湖北武汉430071 [2]湖北省卫生监督局,湖北武汉430079

出　　处：《食品科学》2006年第4期215-219,共5页Food Science

基　　金：湖北省卫生厅攻关课题(2003GX1B135)

摘　　要：目的:探讨番茄汁对人前列腺癌PC-3细胞生长抑制作用及其机理研究。方法:番茄红素及不同浓度番茄汁作用于人前列腺癌PC-3细胞48h;采用生长曲线及噻唑蓝(MTT)法检测番茄汁、番茄红素对人前列腺癌PC-3细胞的生长抑制作用,通过原位(缺口)末端标记法(TUNEL)法观察凋亡细胞的形态结构变化,流式细胞仪检测细胞凋亡峰。结果:番茄汁、番茄红素能显著抑制人前列腺癌PC-3细胞生长;TUNEL方法检测呈阳性;流式细胞仪分析图上出现典型的凋亡细胞峰。以上各指标中,番茄汁随着浓度增大其作用加强;低剂量组的作用虽比番茄红素组差,中剂量组的作用与番茄红素组相比无差别,高剂量组的作用比番茄红素组强。结论:番茄汁对抗人前列腺癌PC-3细胞的作用,除番茄红素外,可能还存在其他抗癌成分的交互作用。番茄汁、番茄红素对PC-3细胞生长抑制作用的机理可能与其诱导人前列腺癌PC-3细胞细胞凋亡有关。Objectives： To study the inhibitory effects of tomato juice on the growth of human prostate carcinoma PC-3 cells and the probable mechanism. Methods： PC-3 cells were treated with the tomato juice in different coneentrations（40, 80, 120 μl/ml） and different contents lycopeue for 4811; the proliferation of PC -3 cells were assayed by growth curve and MTT（Thiazolyl blue） assay. TUNEL（Terminal decxynueleotide mediated nick end labeling） were used to observe the morphology of apoptotic cells. The flow cytometry was also used to analyze the PC-3 apoptosis. Results： High concentrations lycopene and tomato juice can markedly inhibR the proliferation of PC-3 cells, while TUNEL positive cells were detectable. Apoptotic peaks by flow cytometry in experimental groups and lycopene group are also detected. The effects measured in experiments above were enhanced with the increasing of tomato juice. The effects of low dose group are weaker than lycopene group but there is no difference between the middle dose group and the lycopene group.The effect of high dose group is stronger than the lycopene group. Conclusions： This study revealed that the anti- prostate cancer effect of tomato juice is not only related to lycopene but also to, other components which have the synergistical interection with lyeopeue. And lycopene and tomato juice can induce apoptosis of PC-3 cells, a result related to inhibiting the proliferation of PC-3 cells.

关 键 词：番茄汁 番茄红素 人前列腺癌PC-3细胞 生长抑制作用 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] Q539[医药卫生—中医学]