黄杨宁缓释片对急性心肌梗塞犬血清微量元素和心肌形态学的影响  

Effects of Cyclovirobuxinum D sustained-release tablet on serum trace elements and myocardial morphology in acute myocardial infarction dogs

作　　者：薛世泉[1] 吕文伟[2] 徐冰[3] 周莉[4] 纪海婷[4] 孙连坤[3] 

机构地区：[1]吉林大学第一医院病理科,吉林长春130021 [2]吉林大学基础医学院药理学教研室,吉林长春130021 [3]吉林大学基础医学院病理生理学教研室,吉林长春130021 [4]吉林大学

出　　处：《吉林大学学报（医学版）》2006年第3期387-389,F0002,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技发展计划项目资助课题(2005139)

摘　　要：目的:观察黄杨宁缓释片对急性心肌梗塞犬血清酶、微量元素和心肌形态学的影响。方法:取杂种犬30只,随机分为5组(每组6只),分别为模型组、黄杨宁片组及黄杨宁缓释片0.16、0.32和0.64 mg.kg-1剂量组。结扎犬左冠状动脉前降支,制备急性心肌梗塞模型,观察黄杨宁缓释片对血清心肌酶活性及微量元素(Cu2+,Zn2+,Mg2+)含量的影响;通过HE染色,观察心肌细胞形态学的变化。结果:与模型组比较,黄杨宁缓释片各剂量组血清中磷酸肌酸激酶(CPK)、乳酸脱氢酶(LDH)、谷草转氨酶(AST)的活性降低(P<0.05,P<0.01),血清微量元素Cu2+含量降低(P<0.05,P<0.01),Zn2+、Mg2+含量增加(P<0.05,P<0.01),缺血心肌细胞的病理形态学损伤程度减轻。结论:黄杨宁缓释片对实验性心肌梗塞犬的心肌具有明显的保护作用。Objective To examine the effects of Cyclovirobuxinum D Sustained-release tablet on the serum enzymes and trace elements and myocardial morphology in acute myocardial infarction dogs. Methods Thirty heterogeneous dogs were divided into five groups （n= 6）： model group, control group with curative drug, the Cyclovirobuxinum D sustained-release tablet groups with 0.16, 0.32 and 0.64 mg·kg^-1 , respectively. The changes in the activities of the enzymes and the contents of serum trace elements （Cu^2＋ , Zn^2＋ , Mg^2＋） were determined in the myocardial infarction models by ligating the left anterior descending branch （LVD） of dogs. The morphological changes of myocardial cells was observed by using HE staining. Results Compared with model group, the activities of AST, CPK, LDH and the content of Cu^2＋were decreased significantly in the groups with different doses of Cyciovirobuxinum D sustained-release tablet （P〈0.05, P〈0.01）, whereas the contents of Zn^2＋and Mg^2＋ were increased （P〈0.05, P〈0.01）. Consequently, Cyclovirobuxinum D sustained release tablet ameliorated the pathomorphological injury of myocardial cells. Conclusion Cyclovirobuxinum D sustained-release tablet has apparently protective effects on myocardium in canine experimental myocardial infarction models.

关 键 词：黄杨宁缓释片 心肌梗塞 心肌酶 痕量元素 疾病模型 动物 

分 类 号：R-33[医药卫生] R965