杂色云芝漆酶基因在甲醇毕赤酵母中表达条件研究  

STUDIES ON THE CONDITIONS OF THE OVEREXPRESSION OF CORIOLUS VERSICOLOR LACCASE (LCC1) GENE IN PICHIA METHANOLICA

作　　者：郭梅[1,2] 路福平[1] 蒲军[1] 白东清[1,3] 杜连祥[1] 

机构地区：[1]天津科技大学生物工程学院工业微生物重点实验室 [2]天津农学院食品科学系,天津300384 [3]天津农学院水产系水生态重点实验室,天津300384

出　　处：《食品研究与开发》2006年第4期82-84,共3页Food Research and Development

摘　　要：重组甲醇毕赤酵母(Pichiamethanolica)工程菌PMAD16/pMETA-Lcc1能分泌表达杂色云芝重组漆酶。本文通过对其发酵条件的研究,找出瓶发酵表达漆酶的最适培养条件:在BMMY培养基中添加0.2mmol/L的Cu2+,装液量为25mL/250mL三角瓶,接种量体积之比为45:25,0.5%甲醇诱导,20℃条件下瓶培养5d,漆酶最高酶活力达1192U/L。Recombinant Pichia methanolica （PMAD16/pMETA-Lccl） strain can express and secrete recombinant laccase protein. The optimum expression conditions of laccase were studied. It is found that 0.2 mmol/L Cu^2＋ in BMMY, 25 mL culture volumes in 250 mL shake-flasks.The inoculation ration of growth medium to the induction medium was 45：25, 0.5 % （v/v） methanol, at 20 ℃ culture 5th day are the optimum expression condition. The activity of extracellular laccase is up, to 1 192 U/L.

关 键 词：漆酶 重组甲醇毕赤酵母 表达条件 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]