接种S180肉瘤的小鼠肿瘤组织和器官中组蛋白与正常小鼠的比较研究  

Comparative to the Histone Derived from the S180-Transplanted Mice and the Normal Mouse

作　　者：李俊燕[1] 周克夫[1] 何晶[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第B05期146-148,共3页Journal of Xiamen University：Natural Science

基　　金：厦门大学细胞生物学和肿瘤细胞工程教育部重点实验室开放基金(2005108)资助

摘　　要：采用酸抽提法分别提取接种S180肉瘤的小鼠肿瘤组织以及肝、脾、肾等器官的组蛋白和正常小鼠相应组织和器官的组蛋白,并采用SDS-PAGE方法进行检测.结果表明,正常小鼠的肝、脾、肾等器官提取的组蛋白在检测中可以看到组蛋白H1的亚带,而接种了S180肉瘤的小鼠的肿瘤组织以及肝、脾、肾等器官的组蛋白在检测中基本无明显的条带.这说明发生在小鼠身上的S180肿瘤组织对小鼠的肝、脾、肾组织中的组蛋白产生一定影响.The histone Hl is a kind of alkalescence protein, which plays an important role in the expression of gene. The histones from tumor tissues and liver, spleen, kidney of S180-transplanted mice and the same apparatus and tissues of normal mouse were extracted by acid-extraction and detected by SDS-PAGE electrophoresis. The results showed that histone Hl second-strips were detected obviously from normal mouse＇liver, spleen and kidney, but there were not obvious histones from tumor tissues and liver, spleen, kidney of S180-transplanted mice comparatively, which suggest that the tumor tissues of the S180-transplanted mice could affect the historic level of their own apparatus and tissues, such as liver, spleen and kidney.

关 键 词：组蛋白H1 S180肉瘤 酸抽提 SDS-PAGE 

分 类 号：R730.2[医药卫生—肿瘤]