CpG ODN2006对乙型肝炎疫苗免疫BALB/c小鼠抗体产生的影响  

Influence of CpG ODN2006 on Antibody Production in BALB/c Mice Immunized with Hepatitis B Vaccine

作　　者：郑源强[1] 石艳春[1] 杨贵贞[2] 

机构地区：[1]内蒙古医学院分子生物学研究中心 [2]吉林大学医学部基础医学院免疫学教研室,长春130021

出　　处：《中国生物制品学杂志》2006年第3期223-224,共2页Chinese Journal of Biologicals

基　　金：内蒙古自然科学基金项目资助(200508010905).

摘　　要：目的探讨合成含CpG基序的寡脱氧核苷酸(CpGODN2006)对乙型肝炎疫苗增强小鼠特异性抗体产生的效应。方法采用BALB/c小鼠,经后腿胫骨前肌注免疫2次,ELISA法检测血清中抗HBsIgG效价。结果疫苗+CpG组较疫苗组和空白对照组的抗HBsIgG效价明显增高,且维持时间长。结论CpGODN2006对小鼠抗HBsIgG的产生具有明显的增强作用。Objective To explore the influence of synthetic CpG ODN2006 on the production of specific antibody in mice immunized with hepatitis B(HB) vaccine.Methods Divide BALB/c mice into test and control groups and immunize twice with CpG ODN2006+HB vaccine and HB vaccine respectively.Separate murine sera for determination of anti-HBs IgG titers by ELISA.Results Compared with those in control group,the anti-HBs IgG titer of mice in test group was significantly high and persistent.Conclusion CpG ODN2006 enhanced the production of anti-HBs IgG in mice.

关 键 词：CPG ODN 乙型肝炎疫苗 抗体 佐剂 

分 类 号：R57[医药卫生—消化系统] R593.1[医药卫生—内科学]