平菇转化叶黄素酯研究  

Research on biotransformation of lutein esters by Pleurotus.ostreatus

作　　者：彭宏[1] 师舞阳[1] 李凤琦[1] 杨宇[1] 陈龙胜[1] 

机构地区：[1]中南大学资源加工与生物工程学院

出　　处：《现代生物医学进展》2006年第4期8-9,F0003,共3页Progress in Modern Biomedicine

基　　金：国家创新研究群体自然科学基金项目(50321402);湖南省自然科学基金项目(05JJ30023)

摘　　要：目前针对有关天然产物的生物转化研究较少,首次报道了平菇转化叶黄素酯的研究。在对平菇在叶黄素酯培养基生长的观察中,发现对于固体培养基,菌丝体可利用叶黄素酯但不能增生,而孢子悬液不能生长,但在发酵液中两者生长良好,可见只有菌丝体可分泌水解酶,而且该酶在溶液环境下效价高。同时利用高效液相色谱测定其降解效果发现,平菇首先将叶黄素酯转化为叶黄素,同时也可将叶黄素转化为其它物质,故控制发酵时间非常重要,这为今后的工业化提供了基础。With regard to few reports on biotransformation of natural products, this paper firstly study the biotransfonnafion of lutein by Pleurotus.ostreatus（P.ostreatus）. Myeclia can grow by use of lutein ester but not proliferate, and supernatant spores can not grow in terms of P.ostreatus on solid medium while both can grow well on liquid medium. So, only mycelia can secret the cxtracelluar enzyme which is more effective in solution. According to enzymatic hydrolysis of lutein esters by HPLC, lutein esters can be transformed to lutein, then lutein also be transformed other chemical products, so the control of fermentation time is important. These results provide research conditions for future industry application.

关 键 词：叶黄素酯 平菇 高效液相色谱 生物转化 

分 类 号：Q81[生物学—生物工程] O623.624[理学—有机化学]