豆粕水解液为氮源细菌厌氧流加发酵生产L-乳酸  

Production of L-Lactic Acid by Anaerobic Fed-batch Fermentation by Lactic Acid Bacteria with Soybean Meal Hydrolysate as Nitrogen Source

作　　者：丁绍峰[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京市生物加工过程重点实验室,北京100029

出　　处：《过程工程学报》2006年第1期77-81,共5页The Chinese Journal of Process Engineering

基　　金：国家'973'基金资助项目(编号:2003CB716002);国家'863'基金资助项目(编号:2002AA217022)

摘　　要：采用细菌厌氧发酵法生产L?乳酸,由实验确定了最佳接种量、发酵温度和pH调节剂,考察了初始葡萄糖浓度对L?乳酸生产的影响,确定初始糖浓度为70～90g/L时得率、产率、最终生物量分别达到92.68g/g,3.17g/(L?h)和8.5×107mL?1.为进一步降低L?乳酸生产成本,以豆粕水解液为氮源代替酵母粉,同时应用流加发酵技术,L?乳酸产量、得率、产率及转化率分别达到155g/L,95.5g/g,1.64g/(L-h)和96.9%.在保证L?乳酸最终浓度的同时可降低生产成本,为进一步工业化奠定了基础.The L-lactic acid production by anaerobic fermentation of lactic acid bacteria was studied. The effects of fermentation conditions such as inoculation, temperature and pH neutralizer on the fermentation of L-lactic acid were determined. When the initial medium containing 70-90 g/L glucose was used, the maximum L-lactic acid yield （92.68%） on total glucose consumed, L-lactic acid productivity [3.17 g/（L·h）] and the cell final biomass （8.5×10^7 mL^-1） were obtained. A new promising nitrogen source, soybean meal hydrolysate （SMH）, can replace yeast extract （YE） completely in order to reduce the manufacturing cost of lactic acid. The maximum L-lactic acid concentration （155 g/L） in fed-batch culture was obtained besides L-lactic acid yield, productivity and the rate of glucose consumed were up to 95.5 g/g, 1.64 g/（L·h） and 96.9% respectively.

关 键 词：L-乳酸 豆粕水解液 流加发酵 

分 类 号：TQ92[轻工技术与工程—发酵工程]