微生物法生产1,3-丙二醇过程的代谢工程研究进展  

Recent Developments in Microbial Metabolic Engineering for the Production of 1,3-Propanediol

作　　者：王宝光[1] 刘铭[1] 杜晨宇[1] 沈金玉[1] 曹竹安[1] 

机构地区：[1]清华大学化工系生物化工研究所,北京100084

出　　处：《过程工程学报》2006年第1期144-149,共6页The Chinese Journal of Process Engineering

基　　金：国家重点基础研究发展计划(973计划)资助项目(编号:2003CB716007)

摘　　要：代谢工程在微生物发酵法生产化工产品的过程中扮演着越来越重要的角色.本研究以生物法生产1,3?丙二醇所涉及的关键酶、代谢途径及其改造为考察对象,系统综述了微生物法生产1,3?丙二醇所涉及的代谢工程技术、最新应用情况及其进展,并展望了未来的发展趋势.Integration of metabolic engineering and fermentation production technologies is necessary for the successful commercial production of chemicals, For the case of biological production of 1,3-propanediol with glycerol, the natural strains are utilized to produce 1,3-propanediol, but the by-products inhibit the product yield for consuming reducing equivalents, however by means of metabolic engineering, this problem can be solved. Besides, by means of utilizing genes from natural strains that produce 1,3-propanediol from glycerol, a recombinant strain that utilizes the cheaper feedstock glucose can be realized, it will increase the competitiveness of the biological production of 1,3-propanediol, In this article, the metabolic engineering of the glycerol pathway and glucose pathway are reviewed respectively, as well as the recent developments and new trends,

关 键 词：代谢工程 1 3-丙二醇 生物催化剂 

分 类 号：TQ923[轻工技术与工程—发酵工程]