分泌型人胸腺素α_1基因工程菌高密度发酵工艺的研究  

Study on high cell density fermentation of secretory recombinant human thymosin alpha 1

作　　者：林陈水[1] 黎小军[2] 付水星[1] 许明[1] 梅建凤[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310032 [2]江西新余高等专科学校,江西新余338031

出　　处：《浙江工业大学学报》2006年第3期277-280,共4页Journal of Zhejiang University of Technology

基　　金：浙江省科技厅资助项目(No.2005C33031)

摘　　要：探索分泌型重组人胸腺素1α工程菌的高密度高表达发酵工艺.在摇瓶培养获得大肠杆菌分泌型重组人胸腺素1α融合蛋白发酵的最佳条件的基础上,用15 L自控发酵罐进行分批补料培养,发酵中分阶段限制性流加氮、碳源,保持溶氧在30%以上,OD600约为25的时候,添加0.5 mmol/LIPTG于28℃诱导10 h.最终菌体密度OD600可达40以上,目的产物占菌体总蛋白25%以上.分泌型人胸腺素1α基因工程菌高密度发酵工艺的建立为工业化生产重组人胸腺素1α提供基础.To research the high cell density and high-expression fermentation of secretory recombinant human thymosin α1, the fed-batch fermentation was carried out in 15 L automatic fermentor on the basis of the optimized culture conditions obtained from the process of flask shaking culture. During the process of fermentation, nitrogen and carbohydrate were controlled at a special speed. The dissolved oxygen was maintained around 30%. And the induction started as OD600 reached about 25 by adding IPTG to a final concentration of 0.5 mmol/L and then continued incubation 10 hours at 28℃. The final cell density （OD600） exceeded 40, and the recombinant protein expression level was over 25% of the total cellular protein. The study provides a basis for largescale production of human thymosin α1 in industrial fermentation.

关 键 词：高密度发酵 胸腺素Α1 大肠杆菌 表达 

分 类 号：TQ92[轻工技术与工程—发酵工程] Q78[生物学—分子生物学]