全反式维甲酸对脑胶质瘤细胞Cyclin E、Caspase-3表达的影响  

Effects of all-trans retinoic acid on expression of Cyclin E,caspase-3 in glioma cell

作　　者：綦斌[1] 罗毅男[1] 田宇[2] 

机构地区：[1]吉林大学第一临床医学院神经外科,长春130021 [2]吉林大学中日联谊医院神经外科

出　　处：《中华实验外科杂志》2006年第6期721-723,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(30271334);吉林省科技发展计划资助项目(20010533)

摘　　要：目的探讨全反式维甲酸对大鼠C6脑胶质瘤细胞增殖抑制作用及细胞周期素Cy- clin E和凋亡相关基因Caspase-3 mRNA表达的影响。方法噻唑蓝(MTT)比色法检测全反式维甲酸(ATRA)在不同浓度(1、2、4、8、16μmol/L)、不同作用时间(24、48、72 h)处理大鼠胶质瘤C6细胞系后,对细胞增殖抑制率的变化；逆转录-聚合酶链反应(RT-PCR)法检测细胞周期素Cyclin E和Caspase-3 mRNA在24、48、72 h不同时间点表达变化。结果ATRA对C6细胞有增殖抑制作用,具有时间和浓度依赖性；药物作用组与无药物作用组之间及药物作用各组之间的OD值和抑制率比较差异有统计学意义(P＜0．01)。随ATRA作用时间的延长,细胞周期素Cyclin E mRNA表达逐渐下降,与细胞凋亡相关基因Caspase-3 mRNA表达逐渐增高(P＜0．01)。结论ATRA抑制C6脑胶质瘤细胞发生增殖,其机制可能与细胞周期素Cyclin E表达下调,凋亡相关基因Caspase-3上调有关。Objective To study the effects of aU-trans retinoic acid on cell proliferation and expres, sion of the cyclin E, Caspase-3 in rat C6 glioma cells. Methods C6 glioma cells were cultured in vitro with 1, 2, 4, 8 and 16 pmol/L ATRA for 24, 48, 72 h respectively. The rate of inhibition was examined by MTT. The expression of cyclin E and Caspase-3 was detected by RT-PCR. Results MTT showed that ATRA could suppress the proliferation of C6 glioma cells in a dose-and time-dependent manner. There was significant difference in A values and the inhibition rate between ATRA experimental groups and control group （P〈0.01）.The expression of cydin E mRNA was down-regulated and that of Caspase-3 mRNA up-regulated after A TRA treatment （P〈0.01 ）. Conclusion ATRA can inhibit the proliferation of rat C6 cells probably by down-regulating cyclin E and up-regulating Caspase-3 gene.

关 键 词：全反式维甲酸 胶质瘤 CYCLIN E CASPASE-3 

分 类 号：R739.41[医药卫生—肿瘤]