维生素C对糖尿病大鼠血脂和主动脉硫酸肝素蛋白聚糖蛋白表达的影响  

Effect of Vitamin C on Blood Lipid and the Aorta Expreeion of Heparin Sulfate Proteoglycan Protein in Streptozotocin-Induced Diabetic Rats

作　　者：李强翔[1] 文格波[1] 何珊[1] 肖扬[2] 谢小英[2] 欧明贤 

机构地区：[1]南华大学附属第一医院,湖南省衡阳市421001 [2]娄底市中心医院内分泌科,湖南省娄底市417000

出　　处：《中国动脉硬化杂志》2006年第3期197-200,共4页Chinese Journal of Arteriosclerosis

基　　金：湖南省教委资助项目(97B103)

摘　　要：目的观察维生素C对糖尿病大鼠血脂和主动脉硫酸肝素蛋白聚糖表达的影响。方法利用链脲佐菌素腹腔注射法诱导建立1型糖尿病大鼠模型,将实验用SD大鼠随机分正常对照组、糖尿病组、维生素C治疗组。治疗16周,观察治疗期间及实验后大鼠的一般状况、血糖、甘油三酯、总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、糖化血红蛋白、糖化低密度脂蛋白,分离主动脉,HE染色及免疫组织化学检测主动脉内膜硫酸肝素蛋白聚糖表达。结果①造模4组大鼠均出现血脂异常及主动脉病理形态改变。②维生素C对血糖无影响,但能改善基本状况,维生素C能降低糖尿病大鼠的甘油三酯(P<0.05)、总胆固醇(P<0.05)、低密度脂蛋白胆固醇(P<0.05)、糖化血红蛋白(P<0.05)、糖化低密度脂蛋白(P<0.05),升高高密度脂蛋白胆固醇(P<0.05);维生素C增强主动脉硫酸肝素蛋白聚糖(P<0.05)表达。结论维生素C无降糖作用,但具有确切的主动脉保护作用。Aim To observe the effect of vitamin C on blood lipid and aorta expreeion of heparin sulfate proteoglycan protein （HSPG） in streptozotecin-induced diabetic rats. Methods Diabetic nephropathy rats were induced by intraperitoneal injection of STZ. Rats were allocated to normal control group, model group, and vitamin C group for 16 weeks. During and after the treatment, during and after the treatment, Its general state, blood suger （BS）, triglyceride （TG）, total cholesterol （TC）, low density lipoprotein （ LDLC）, high density lipoprotein （ HDLC）, glycosylated hemoglobin （HbAlc） and glycosylated low density lipoprotein （ G-LDL） Alteration of tissue morphology via HE staining was observed after the aorta was taken out from the rats executed by narcosis; heparin stdfate proteoglycan protein, were determined by immunohistechemisteche. Results （1） Diabetes mellitus and aortalesion occurred in the four model groups. （2）Vitamin C have no effect on the blood gloucoselevels, while they could improve the general state, improve the general state, decreasetriglyceride （P〈0.05 ）, serum cholesterol （ P〈0.05 ）, low density lipoprotein （ P〈0.05 ）, glycosylated hemoglobin （ P〈0.05 ）, and glycated low density lipoprotein （ P〈0.05 ）, increase high density lipoprotein （ P〈0.05 ）, Theexprecion of HSPG in the kidney were significantly inhibited （ P〈0.05 ） at the same time. Conclusion Vitamin C have no effect in decreasing the blood sugar level but have a certain in preventing renal.

关 键 词：病理学与病理生理学 维生素C 糖尿病模型 血脂 主动脉 硫酸肝素蛋白聚糖蛋白 

分 类 号：R363[医药卫生—病理学]