烟酸对冠心病患者外周血单核细胞环一磷酸腺苷与三磷酸腺苷结合盒转运体A1表达的影响  

The Correlative Study on the Expression of Cyclic Adenosine Monophotosphate and Adenosine Triphosphate Binding Cassette Transporter A1 in Peripheral Blood Mononuclear Cells by the Role of Niacin

作　　者：李颖庆[1] 文红艳[1] 全智华[1] 李华波[1] 杨薪[1] 

机构地区：[1]南华大学附属第一医院心血管内科,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2006年第3期208-212,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的以冠心病患者的外周血单核细胞为研究对象,观察烟酸对体外培养的单核细胞中环一磷酸腺苷及ATP结合盒转运体A1表达的影响,分析烟酸调节ATP结合盒转运体A1的表达与环一磷酸腺苷—蛋白激酶A途径的相关性。方法运用逆转录聚合酶链反应和Westernblot蛋白印迹分别检测ATP结合盒转运体A1mRNA与ATP结合盒转运体A1蛋白的表达,采用低pH值EIA法测定细胞内环磷酸腺苷水平。结果(1)各组外周血单核细胞中ATP结合盒转运体A1的表达及环一磷酸腺苷的水平在3h和48h的变化无明显差异(P>0.05);(2)冠心病高血脂患者单核细胞中ATP结合盒转运体A1的表达较正常人明显降低(P<0.05),而正常血脂组单核细胞中ATP结合盒转运体A1的表达较正常人无明显差异(P>0.05);(3)烟酸单独作用能明显增加冠心病人单核细胞中ATP结合盒转运体A1的表达(P<0.05),同时使单核细胞中环一磷酸腺苷的含量增加(P<0.05);(4)蛋白激酶A抑制剂Ro31-8220单独作用能明显降低冠心病人单核细胞中ATP结合盒转运体A1的表达(P<0.05),同时使单核细胞中环—磷酸腺苷的含量降低(P<0.05);(5)先用Ro31-8220作用30min后,再加入烟酸,则烟酸使单核细胞中ATP结合盒转运体A1表达上调及环一磷酸腺苷的含量增加的作用被完全抑制(P<0.05)。结论烟酸对单核细胞中ATP结合盒转运体A1表达的影响,是通过环一磷酸腺苷-蛋白激酶A途径发挥作用的,烟酸上调外周血单核细胞中ATP结合盒转运体A1表达的作用,与环一磷酸腺苷—蛋白激酶A途径存在明确的相关性。Aim Take coronary heart disease patient＇s peripheral blood mononuclear cells （PBMC）as the study object, observing the effect of niacin to the expression of cyclic adenosine monophotosphate （ cAMP ） and adenosine triphosphate binding cassette transporter AI （ABCAI） in PBMC which incubated in vitro, analysing the correlation of niacin adjusts the expression of ABCAI and cAMP/ protein kinase A （PKA） pathway. Methods To detect the expression of ABCAI mRNA and protein by reverse trancriptase-polymerase chain reaction （RT-PCR） and western blot, to determine the level of cAMP in PBMC by low pH EIA kit. Analysing the expression of ABCAI change and cAMP level change, discuss the correlation on the expression of ABCAI and cAMP in PBMC by the role of niacin. Results （ 1 ）Each group of the expression of ABCAI and the cAMP level changes in PBMC in 3 h and 48 h does not have the obvious difference （ P〉0.05 ） , （2） The expression of ABCAI of coronary heart disease high blood fats patient in PBMC compares the normal person obviously to reduce （ P〈0.05 ）, but in the normal blood fats group the expression of ABCAI in PBMC compares the normal person not to have the obvious change （ P〉0.05 ）. （ 3 ） The niacin alone affects obviously increase in coronary heart disease person uninuclear cell the ABCAI expression （ P〈0.05）, at the same time makes the cAMP content in the PBMC to increase （P〈0.05 ）. （4） PKA inhibitor Ro 31-8220 alone affects can obviously reduce in coronary disease person he expression of ABCAI in PBMCs （P〈0.05 ）, at the same time causes the cAMP content in PBMCs to reduce （P〈0.05 ）. （ 5 ） In the niacin ＋ PKA inhibitor group were preincubated for 30 minutes in the presence of PKA inhibitor Ro 31-8220 （900 nmol/L）, before niacin （ 1 000μmol/L） was added, then the niacin increases the expression of ABCA1 and the cAMP content in PBMCs was suppressed completely （ P〈0.05 ）. Conclusion According to the result analysis of the ex

关 键 词：病理学与病理生理学 烟酸 细胞培养 单核细胞 三磷酸腺苷结合盒转运体A1 环一磷酸腺 苷 蛋白激酶A抑制物 冠状动脉疾病 

分 类 号：R363[医药卫生—病理学]