Phenylacetate Down-regulates Expression of RNA Editing Deaminase ADAR2 mRNA in U-251MG Glioma Cells  

作　　者：Yu Tian Yuzuo Pan Yufei Gao Guilin Li Jun Gao Xingli Zhao Guiying Li Renzhi Wang 

机构地区：[1]Department of Neurosurgery, Sino-Japanese Friendship Hospital, Jilin University, Changchun 130033, China [2]Department of Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China [3]Department of Neurosurgery, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100005, China [4]Key Laboratory for Molecular Enzymology ＆ Engineering, the Ministry of Education, Jilin University, Changchun 130021, China

出　　处：《Chinese Journal of Clinical Oncology》2006年第1期1-4,共4页中国肿瘤临床（英文版）

基　　金：This study was supported by the National Nature Science Foundation of China (No. 30271334).

摘　　要：OBJECTIVE To observe the effect of phenylacetate on the expression of RNA editing deaminase ADAR2 mRNA in glioma cells. METHODS Primary glial cells from human brain tissue and glioma U-251MG cells were cultured. The expression of ADAR2 mRNA was detected by a semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The levels of ADAR2 mRNA expression before and after treatment with phenylacetate were tested by RT -PCR and image analysis. The level of ADAR2 gene expression was presented as the ratio expression rate (RER) of ADAR2 gene/β-actin based on computer image analysis. RESULTS The ADAR2 mRNA displayed mild expression in brain glial cells, and a high expression level in high-grade malignant U-251MG glioma cells. Computer image analysis showed that the RERs of the ADAR2 gene in the U-251MG cells before and after treatment with 4.0, and 5.0 mM phenylacetate for 8 h were 100.0, 73.5, 60.3, respectively. The expression of ADAR2 mRNA was decreased by phenylacetate in glioma U-251MG cells. CONCLUSION Phenylacetate can decrease the expression of ADAR2 mRNA in glioma cells, suggesting that phenylacetate, as a drug, may act on the course of RNA editing in gliomas.OBJECTIVE To observe the effect of phenylacetate on the expression of RNA editing deaminase ADAR2 mRNA in glioma cells. METHODS Primary glial cells from human brain tissue and glioma U-251MG cells were cultured. The expression of ADAR2 mRNA was detected by a semiquantitative reverse transcription-polymerase chain reaction （RT-PCR）. The levels of ADAR2 mRNA expression before and after treatment with phenylacetate were tested by RT-PCR and image analysis. The level of ADAR2 gene expression was presented as the ratio expression rate （RER） of ADAR2 gene/β-actin based on computer image analysis. RESULTS The ADAR2 mRNA displayed mild expression in brain glial cells, and a high expression level in high-grade malignant U-251MG glioma cells. Computer image analysis showed that the RERs of the ADAR2 gene in the U-251MG cells before and after treatment with 4.0, and 5.0 mM phenylacetate for 8 h were 100.0, 73.5, 60.3, respectively. The expression of ADAR2 mRNA was decreased by phenylacetate in glioma U-251MG cells. CONCLUSION Phenylacetate can decrease the expression of ADAR2 mRNA in glioma cells, suggesting that phenylacetate, as a drug, may act on the course of RNA editing in gliomas.

关 键 词：GLIOMA ADAR2 RT-PCR. 

分 类 号：R730.264[医药卫生—肿瘤]