树突状细胞在黏膜免疫模型小鼠中的分布及E-cadherin和ICAM-1表达的研究  

THE STUDY ON THE DISTRIBUTION OF DENDRITIC CELLS AND EXPRESSIONS OF E-CADHERIN AND ICAM-1 IN MUSCOSAL IMMUNE MOUSE MODEL

作　　者：方传龙[1] 谢遵江[1] 贾立敏[1] 贺业春[1] 刘颖[1] 刘丽[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,哈尔滨150086

出　　处：《解剖学报》2006年第3期332-336,共5页Acta Anatomica Sinica

基　　金：黑龙江省自然科学基金资助项目(D0234)

摘　　要：目的通过研究小鼠树突状细胞在黏膜免疫模型小鼠中分布特征及上皮型钙黏附分子(E-cadherin)和细胞间黏附分子-1(ICAM-1)的表达,以探讨E-cadherin和ICAM-1在树突状细胞迁移过程中的调控作用。方法采用光镜和免疫组织化学染色方法,观察黏膜免疫模型小鼠中树突状细胞的分布特征;分析E-cadherin和ICAM-1的表达情况。结果体内的树突状细胞在黏膜免疫模型小鼠中,主要分布于肠系膜淋巴结、回肠集合淋巴小结、胃和空回肠黏膜及黏膜下层,与对照组相比有显著差异,其ICAM-1表达明显增高,E-cadherin表达下调,分别与对照组数密度和面密度比较有显著差异。结论在树突状细胞迁移运动过程中,E-cadherin和ICAM-1黏附分子可能起着关键性的调控作用。Objective To study the distributive characteristics of DC and the expressions of E-cadherin and ICAM-1 in muscosal immune mouse model, to discuss the regulative role for E-cadherin and ICAM-1 in the DC migration. Methods By the method of immunohistochemistry staining and light microscopy, we observed the distributive characteristics of DC in muscosal immune mouse model, analysed the expressions of E-cadherin and ICAM-1. Results The DC mainly distributed in mesenteric lymph node （MLN）, Peyer＇s patch（PP）, and GUT （stomach, jejunum and ileum） of muscosal immune mouse model. The number of DC was larger in the experiment group than that in control group. The expression of ICAM-1 was higher, but lower of E-cadherin in muscosal immune mouse model. The number and area density of experiment group were greater compared with that of control group. Conclusion The E-cadherin and ICAM-1 may play a critical role in DC migration.

关 键 词：树突状细胞 上皮型钙黏附分子 细胞间黏附分子-1 免疫组织化学 黏膜免疫小鼠模型 

分 类 号：R392.12[医药卫生—免疫学]