榄香烯对小鼠瘤株Hca-A2/p化疗的影响  

Effect of elemene in chemotheapy of mouse hepatocarcinma Hca-A2/p

作　　者：高海德[1] 张佩禹 赵荣宇[3] 陈宏生[1] 邢状杰[1] 柳宗林[1] 

机构地区：[1]大连大学附属中山医院普外一科,辽宁大连160001 [2]大石桥市中心医院普外科,辽宁大石桥115001 [3]大连医科大学附属第一医院普外二科,辽宁大连160027

出　　处：《中华肿瘤防治杂志》2006年第7期492-494,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:观察榄香烯对小鼠肝细胞癌的治疗作用,为临床肝癌的治疗提供理论依据。方法:做荷瘤小鼠动物模型,癌瘤内多点多次注射给药,用RT-PCR方法测其多药耐药基因(multidrugs resistancegene,mdr1)的表达,同时观察抑瘤率、肿瘤坏死情况和小鼠死亡率。结果:荷瘤组(LT)、化疗组(C)、榄香烯组(E)和化疗药+榄香烯组(CE)的mdr1分别为(92·9%、100·0%、14·3%和14·3%),其中E、CE与LT的mdr1比较,P<0·05;LT++(42·9%)与C++(85·7%)的mdr1比较P<0·05。C、E和CE抑瘤率分别为(35%、27·2%和41·8%)与LT比较P<0·05。C、E和CE的死亡率分别为(15%、10%和5%)与LT(40%)比较P<0·05。病理示CE肿瘤坏死明显,大体见肿瘤腐肉状,色晦暗,质软,小鼠生存活跃。结论:瘤株Hca-A2/p的mdr1的自然表达率为92·9%。化疗使mdr1强表达增加。榄香烯可降低mdr1的表达并有良好的抗肿瘤的作用,与化疗药合用抗肿瘤作用更佳。OBJECTIVE：To offer basic theory for clinical liver carcinoma treatment by observing the therapeutic effects of Elemene in loading tumor mice. METHODS： The pharmaceuticals were injected into tumor of loading tumor mice in different points for many times, and then, determining expression of multidrugs resistance gene （mdr1） by RT-PCR, noticing inhibition rate of tumor, pathol- ogy of tumor and of mortality mice. RESULTS： Expressions of mdrl in loading turner group （LT）, cbemiotberapy group （C）, Elemene group （E）, cbemiotberapy ＋ Elemene group （CE） were 92.9 %, 100.0 %, 14.3%, 14.3 %, respectively and there was a statistical significance between E （or CE） and LT, P〈0.05, between LT ＋＋ and C ＋＋, P〈0. 05. Inhibition rates of tumor of C,E, CE were 35% ,27.2% ,41.8%, respectively and there was a statistical significance, compared with LT, P〈0.05. The mouse＇s mortalities of C, E, CE were （15%, 10%, 5%） respectively and there was a statistical significance, compared with LT（40%）, P〈0. 05. The tumor＇s necrosis was outstanding and the mice were more energetic than others in CE. CONCLUSIONS.. The innate expression of mdrl of Hca-A2/p is 92.9%. The over expression of mdrl of Hca- A2/p increased with chemotherapy. Elemene can reverse mdrl gene and it is effective in killing tumor by itself and more effective by combining it with chemotherapeutic agents.

关 键 词：肝肿瘤/药物疗法 油类 挥发 基因 MDR 小鼠 

分 类 号：R735.7[医药卫生—肿瘤]