The Effect of Tryptophan on Serotonin-Like Neurons in Duck Cerebellum  

作　　者：LIU Hua-zhen TANG Wen-hua PENG Ke-mei CHEN Wen-qin LUO Guan-zhong WANG Yan WEI Lan 

机构地区：[1]College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P.R.China

出　　处：《Agricultural Sciences in China》2006年第6期473-477,共5页中国农业科学（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(39970547);Natural Science Fund of Hubei Province(98J012).We greatly appreciate Academician Chen Huanchun,the vice president of Huazhong Agricultural University,China,for his help and approval on this study.

摘　　要：Healthy Cherry Valley ducks were used in the present study. Different doses of tryptophan were injected intraperitoneally to them after being fasted 4 h （8：00 a.m.-12：00 a.m.）. One hour later, they were deeply anaesthetized and perfused. The cerebellum was removed to make serial paraffin longitudinal sections. The streptavidin-biotin-peroxidase complex （SABC） method was used to study the distribution of serotonin-like neurons in the cerebellum. All films were analysed by using a computer-assisted image analysis system. Serotonin-like neurons are only localized in cerebellar Purkinje cell layer. The optical density averages of serotonin-like neurons in 200 and 100 mg kg^-1 group are significantly higher than that of 0 mg kg^-1 group （P〈0.01）. These results show that serotonin-like neurons are distributed in Purkinje cell layer and that excessive tryptophan can affect the content of serotonin in cerebellum.Healthy Cherry Valley ducks were used in the present study. Different doses of tryptophan were injected intraperitoneally to them after being fasted 4 h （8：00 a.m.-12：00 a.m.）. One hour later, they were deeply anaesthetized and perfused. The cerebellum was removed to make serial paraffin longitudinal sections. The streptavidin-biotin-peroxidase complex （SABC） method was used to study the distribution of serotonin-like neurons in the cerebellum. All films were analysed by using a computer-assisted image analysis system. Serotonin-like neurons are only localized in cerebellar Purkinje cell layer. The optical density averages of serotonin-like neurons in 200 and 100 mg kg^-1 group are significantly higher than that of 0 mg kg^-1 group （P〈0.01）. These results show that serotonin-like neurons are distributed in Purkinje cell layer and that excessive tryptophan can affect the content of serotonin in cerebellum.

关 键 词：TRYPTOPHAN SEROTONIN CEREBELLUM Cherry Valley duck streptavidin-biotin-peroxidase complex （SABC） 

分 类 号：S834[农业科学—畜牧学]