Survivin反义核酸对SMMC-7721细胞增殖和凋亡的影响  

Antisense Oligonucleotide Targeting Survivin Affects the Proliferation and Apoptosis of SMMC-7721 Cells

作　　者：潘克俭[1] 刘小菁[2] 杨春蕾[3] 王玉明[1] 王西岩[1] 

机构地区：[1]成都医学院基础部,成都610083 [2]四川大学华西医院心血管疾病研究室,成都610041 [3]四川大学生命科学院,成都610041

出　　处：《生物医学工程学杂志》2006年第3期625-629,共5页Journal of Biomedical Engineering

摘　　要：Surv iv in是凋亡抑制蛋白(IAP)家族的一个成员,具有强大的抗细胞凋亡功能,在几乎所有肿瘤组织中特异性表达,而在正常成年终末分化组织中低表达甚至不表达。本研究针对Surv iv in mRNA序列设计了反义寡核甘酸,RT-PCR检测表明,该序列反义寡核苷酸可明显降低细胞中surv iv in基因的mRNA含量;W estern印迹显示Surv iv in蛋白水平也被降低。M TT比色实验法检测结果说明人Surv iv in反义寡核苷酸抑制SMM C-7721细胞增殖,抑制率为43%,远高于无义寡核苷酸组和空白对照组。反义寡核苷酸还显著增强SMM C-7721细胞对于抗肿瘤药高三尖杉酯碱的敏感性,TUNEL法检测结果显示,在较低高三尖杉酯碱浓度下,反义寡核苷酸转染细胞的凋亡率明显高于其它对照组。本研究结果提示,surv iv in表达的靶向抑制有望应用于肿瘤的辅助治疗之中。Survivin is a member of inhibitor of apoptosis （IAP） family which has strong ability of antiapoptosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers in vivo, but it is undetectable in terminally differentiated adult tissues. We designed a human survivin antisense oligonucleotides. RT-PCR and Western blot clearly showed that survivin mRNA and protein were decreased by this antisense oligonucleotide. The inhibition of SMMC-7721 cell proliferation was demonstrated by MTT assay. The inhibition rate （43.28 % ± 3.65 % ） was much higher than the inhibition rate （6.76 % ± 0. 92% ） of the control group. The apoptosis rate （33.68% ± 2.89%） obtained by transfecting cells with the use of antisense oligonucleotide was higher than the apoptosis rate （14. 12% ± 1.24%） of the control group. And the result of TUNEL revealed that, when SMMC-7721 cells were exposed to homoharringtoine at low concentration and were transfected by antisense oligonucleotide, the apoptosis rate of the cells was significantly higher than that of other control group. These results indicate that antisense oligonucleotide may have the potential for selective tumor therapy in future.

关 键 词：存活素 反义寡核苷酸 SMMC-7721细胞 增殖 凋亡 

分 类 号：R73-3[医药卫生—肿瘤]