膳食纤维与肌醇六磷酸对1,2-二甲肼诱导大鼠大肠癌发生的作用(英文)  

Effects of dietary fiber and inositol hexaphosphate on the onset of carcinoma of large intestine in rats induced by 1,2-dimethylhydrazine

作　　者：宋扬[1] 张海平[2] 张铮[3] 陈炳卿[1] 

机构地区：[1]哈尔滨医科大学营养与食品卫生学教研室,黑龙江省哈尔滨市161000 [2]青岛市立医院营养科,山东省青岛市266011 [3]青岛大学医学院生物化学教研室,山东省青岛市266021

出　　处：《中国临床康复》2006年第24期178-181,F0003,共5页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:随着国内人民生活水平的不断提高,大肠癌的发生率不断提高。大量的流行病学调查表明,膳食因素与大肠癌的高发有着密切的相关性。目的:通过建立大鼠大肠癌的动物模型,观察膳食纤维与肌醇六磷酸(植酸)对大肠癌发生的作用。设计:随机区组设计。单位:青岛大学医学院营养学研究所。材料:实验于2004-03/12在青岛大学医学院营养学研究所进行。将86只4周龄雄性Wistar大鼠按体质量随机区组分为纤维素组(14只)、果胶组(14只)、植酸组(15只)、纤维素+植酸组(14只)、果胶+植酸组(14只)和对照组(15只)6组。方法:对照组:无膳食纤维的基础饲料;果胶组:添加10%的果胶;纤维素组:添加10%的纤维素;植酸组:添加2%的植酸钠饮水;果胶+植酸组:添加10%的果胶和2%的植酸钠饮水;纤维素+植酸组:添加10%的纤维素和2%的植酸钠饮水。对86只大鼠用1,2-二甲肼皮下注射诱发大肠癌,观察大鼠大肠肿瘤的发生率、肿瘤的数量及体积;测定大鼠大肠粘膜细胞的增殖活性(增殖细胞核抗原阳性细胞数/计数的细胞核总数)。主要观察指标:①各组大鼠大肠肿瘤的发生率,平均每只鼠的肿瘤个数和肿瘤体积变化。②大鼠大肠黏膜细胞的增殖活性。结果:①各组大鼠多死于喂养20周前。果胶组、果胶+植酸组及对照组各有1只大鼠死于喂养20周后。②各组大鼠大肠肿瘤的发生率与对照组相比差异无显著性(P>0.05),但植酸组平均每只鼠的肿瘤个数和肿瘤体积显著低于对照组数量:(1.1±0.2),(4.1±1.2)个/只,P<0.01;体积:(176.1±65.5),(1046.7±469.0)mm3,P<0.05,果胶组、果胶+植酸组平均每只鼠的肿瘤个数显著高于对照组(7.5±1.9),(7.2±1.0)个/只,P<0.05。③植酸组大鼠大肠黏膜细胞的增殖活性比对照组显著降低,果胶组大鼠大肠黏膜细胞的增殖活性比对照组升高(41.8±4.7)%,(83.6±2.9)%,(66.7±7.8)%,P<0.01和0.05。结论:膳食中添加果�BACKGROUND： Incidence rate of carcinoma of large intestine increases with the development of national living standard. Many epidemiologic surveys have showed that dietary factors have closely correlation with high risk of carcinoma of large intestine. OBJECTIVE： To observe the effect of dietary fiber and inositol hexaphosphate on the incidence of carcinoma of large intestine by establishing rat models with carcinoma of large intestine. DESIGN： Randomized block design. SETTING： Institute of Nutriology, Medical College, Qingdao University. MATERIALS： The experiment was performed at the Institute of Nutriology, Medical College, Qingdao University from March to December 2004. Totally 86 male Wistar rats of 4 weeks were randomly assigned based on body mass into cellulose group with 14 rats, pectin group with 14 rats, inositol hexaphosphate group with 15 rats, cellulose plus inositol hexaphosphate group with 14 rats, pectin plus inositol hexaphosphate group with 14 rats and control group with 15 rats. METHODS： Basic feed without dietary fiber was given in the control group. 10% pectin was added in the pectin group. 10% cellulose was added in the cellulose group. 2% sodium phytate was added in the water of inositol hexaphosphate group. 10% pectin and 2% sodium phytate were added in the water of pectin plus inositol hexaphosphate group. 10% cellulose and 2% sodium phytate drinking water were added in the cellulose plus inositol hexaphosphate group. A total of 86 rats received 1,2-dimethylhydrazine （DMH） by subcutaneous injection to induce carcinoma of large intestine at week 4 after breeding. Incidence rate of large bowel neoplasm, number and volume of tumor were observed. Proliferating activity of large intestinal mucosa cells was determined （the ratio of number of positive cells of proliferating cell nuclear antigen to total count of nucleus）. MAIN OUTCOME MEASURES： ①Incidence rate of large bowel neo- plasm in each group, changes of mean number and mean volume of tumors of each rat,

关 键 词：肠肿瘤 植酸 膳食纤维 纤维素 果胶类 

分 类 号：R735.3[医药卫生—肿瘤]