培养细胞牵张损伤装置及测试系统的研制与应用  

Development and application of a device for stretch-induced injury to cultured cell and testing system

作　　者：尹志勇[1] 王正国[1] 蒋建新[1] 杨志焕[1] 周萍[1] 王克万[1] 麻晓林[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第四研究室创伤,烧伤与复合伤国家重点实验室车辆/生物碰撞安全重庆市重点实验室,重庆400042

出　　处：《第三军医大学学报》2006年第13期1363-1366,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(39630330)~~

摘　　要：目的为从细胞水平直接观察应力、应变作用后细胞形态和功能的改变,研制计算机控制的培养细胞牵张损伤装置和相应的测试系统。方法应用空气动力学原理,采用微型空气压缩机、电磁阀、压力传感器、程控放大器和模数转换多功能卡研制了体外培养细胞牵张损伤装置,用于实验研究。其致伤参数由计算机精确控制,并采用压力法获取细胞在致伤过程中的应变规律。对培养的新生大鼠大脑皮层星型胶质细胞应用本致伤装置采用不同的条件致伤,检测乳酸脱清酶(LDH)活性和台盼蓝含量,并观察其形态学的改变。结果采用不同的致伤参数可复制出轻、中、重度的细胞损伤模型。结论培养细胞牵张损伤装置及测试系统的各项性能指标均达到设计要求,致伤过程简便、重复性好、结果稳定。新的乳胶膜变形规律检测方法和装置,明显优于国外文献报道的图像处理检测法,满足实验研究的需要。Objective To develop a device for stretch-induced injury to cultured cells and measure the injury parameters. It makes possible to observe the changes of cell configuration and function directly after injury. Methods The device was developed based on aero-dynamical principle, and consisted of mini-type air compressor, electromagnetic valve, pressure transmitter, PGA amplifier, A/D converter and computer. The computer can accurately control the injury parameters. A new method and device were first introduced to measure the deformation rate and deformation degree of silastic membrance in the stretch induced cultured cell injury process. The device controlled by the computer was used to cause the astrocyte injury. The degree of cell injury was assessed qualitatively by electron microscopy, and quantitatively by lactate dehydrogenase （LDH） enzyme release and trypan blue staining. Results The experiment results showed that the injury of different levels could be reproduced by our device under the injury parameters we set. Conclusion The injury device and the measuring system meet the requirements of our design. The results suggested that the new device and the method were much better than those reported in foreign literature. It has the advantages of simple and convenient manipulation, high precision and real-time processing. It can be used for the cell-level experiment research.

关 键 词：培养细胞 牵张损伤 模型 

分 类 号：R-331[医药卫生] R312