脂肪酶产生菌的筛选及一株黑曲霉产脂肪酶最适条件研究  

Selection of Lipase-Producing Strains and Optimal Lipase Producing Conditions for Aspergillus niger

作　　者：任丽虹[1] 周应揆[1] 

机构地区：[1]云南大学生物系

出　　处：《工业微生物》1996年第1期23-26,共4页Industrial Microbiology

摘　　要：通过固体平板筛选,从520株真菌中分离得到194株产脂肪酶菌。再选择其中透明圈较大的43株菌进行液体复筛,得到产酶活力最高的一株菌为黑曲霉L_1。最后,确定了此菌的最适产酶条件为:橄榄油作碳源,酵母膏作氮源,pH5.8,28℃培养4天。194 lipase-producing strains were selected from 520 fungi through solid plate assay method. A high lipase-yielding strain of Aspergillus niger L1 was obtained by liquid reselection from 43 strains with larger transparent cycles. In addition, the optimal lipase producing conditions of strain L1 were as follows; olive oil as carbon source, yeast as nitrogen source, pH 5.8, 28癈 and the culture period of 96 hours.

关 键 词：脂肪酶 黑曲霉 最适产酶条件 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ925.6