电击法介导的紫孢侧耳原生质体转化  

Transformation of Pleurotus sapidus Protoplasts by Electroporation

作　　者：燕克勤[1] 朱宝成[1] 赵会良[2] 李庆余[1] 王谦 

机构地区：[1]河北大学生物系,保定071002 [2]河北大学静电研究所 [3]河北省徽生物研究所,保定071051

出　　处：《生物工程学报》1996年第1期40-44,共5页Chinese Journal of Biotechnology

摘　　要：使用基因脉冲导入仪成功地将糙皮侧耳DNA导入紫孢侧耳单核原生质体内,获得了具有“锁状联合”特征的双核转化菌株T_1和T_2。转化率为8.2×10^(-5),转化比为3.6％。酯酶同Ⅰ酶分析结果表明,转化菌株除具有受体菌的酶带外,还存在供体菌的酶带,由此证明转化菌株确为紫孢侧耳和糙皮侧耳DNA重组的产物。转化菌株子实体形态也发生了变化。两菌株子实体均不释放孢子;T_1菌柄中生;T_2成熟子实体菌盖中部易长出菌丝。The total DNA of Pleurotous ostreatus was successfully transferred into protoplasts of monokaryotic mycelia of P. sapidus by electroporation. The transformants were selected by their clamp connecton of dikarycocyte, and the transformation frequency and transformation rate were 8-2 x 10 5 and 3-6% respectively. Analysing the es-terase isozyme, it was found that the transformants had some isozyme bands of P. ostreatus and P. sapidus, and new non-parental bands. This proved that the recombination occurred between DNA of P. sapidus and DNA of P. ostreatus. The fruit bodies of the transformants showed changes in their morphological characters.

关 键 词：侧耳 食用菌 原生质体 电击法 转化 

分 类 号：S646.190.3[农业科学—蔬菜学]