HBV－NC1株X基因在真核细胞中表达，基因调控与致癌作用的初探  

Study on the Expression in Eukaryote Cell ,Gene Regulation Effect and Carcinogenesis of the HBV X Gene

作　　者：曾明[1] 黄秉仁[1] 蔡良琬[1] 潘国宗[1] 

机构地区：[1]中国协和医科大学,中国医学科学院基础医学研究所,医学分子生物学国家重点实验室,北京协和医院

出　　处：《生物化学杂志》1996年第1期27-30,共4页

摘　　要：报道了ＨＢＶ－ＮＣ１株的Ｘ基因进入真核细胞表达载体ｐＸＴ１质粒的ＴＫ启动子之后，转染细胞及表达Ｘ基因成功。即发现包装病毒感染ＮＩＨ／３Ｔ３细胞后有Ｘ基因表达，用斑点杂交又证明此Ｘ基因已整合到细胞的基因组中，当与有ｐＭＳＨ报告基因的质粒共同感染真核细胞后确有激活转录调控现象出现。应用反义的ＸＲＮＡ表达载体感染肝癌细胞发现有接触抑制作用出现。The X gene of HBV-NCl DNA was fused with the TK promoter of the eukaryote cell expression plasmid PXT-1 and the recombinant plasmid was successfully transformed into mammalian cell and the X protein was expressed. Using this recombinant plasmid and another plasmid containing luciferase report gene in a cotransfecting experiment,the transcription-stimulating effect was observed. The growth-inhibited action of antisense RNA of X gene was studies here for the first time by using pXT1 -RX pseudovirus transfected hepatoma cell lines.

关 键 词：肿瘤 乙型肝炎病毒 X基因 基因调控 病理 

分 类 号：R730.21[医药卫生—肿瘤]