柔红霉素诱导Jurkat细胞凋亡、抑制增殖与bcl-2、PCNA表达变化的关系  

Daunorubicin induce apoptosis and inhibit proliferation in Jurkat cell lines and its molecular mechanisms

作　　者：李忠俊[1] 陈幸华[1] 

机构地区：[1]第三军医大学新桥医院,重庆400037

出　　处：《重庆医学》2006年第12期1059-1060,共2页Chongqing medicine

摘　　要：目的探讨柔红霉素（DNR）在体外诱导Jurkat细胞凋亡的情况并探讨其与细胞表达凋亡相关蛋白改变的关系。方法Annexin V／PI双标流式细胞仪（FCM）检测DNR诱导Jurkat细胞的凋亡作用，用免疫细胞化学观察相关蛋白表达水平的变化。结果当DNR为0．1～2．0μmol／L浓度范围时，作用一定时间后Jurkat细胞发生的凋亡率随药物浓度的增加与作用时间的延长而升高。但药物浓度超过2．0μmol／L，或2．0μmol／L作用48h后Jurkat细胞出现凋亡率下降，细胞大部分死亡。0．5μmol／L DNR作用于Jurkat细胞24h后，细胞中bcl-2、PCNA蛋白表达水平降低。结论一定浓度的DNR在体外可诱导Jurkat细胞凋亡，药物浓度达5．0μmol／L时细胞大部分死亡。本实验条件下DNR体外诱导Jurkat发生凋亡的机制可能是通过抑制bcl-2、PCNA蛋白的表达实现。Objective To study the effect of daunorubicin on Jurkat cell line apoptosis induction in vitro and its associated mechanisms. Methods The apoptosis of Jurkat cell line was detected by Annexin V/PI in flow cytometry（FCM） hcl-2, PCNA protein expression was observed by immunocytochemistry. Results Treated with 0.1-2.0μmol/L DNR, the percentage of apoptotic Jurkat cells increased following the drug dose as well as the time extending. Whereas,the percentage of apoptotic Jurkat cells was decreased and cells died absolutely when the drug dose was over 2.0μmol/L or cells were treated 48h with 2.0μmol/L DNR. It was showed that bcl-2,PCNA protein expression level of Jurkat cells treated with 0. 5μmol/L DNR for 24h were lower than that of without drug. Conclusion Low dose DNR can efficiently induce apoptosis of Jurkat cell line in vitro, bcl-2 and PCNA gene participate the progress of DNR inducing apoptosis of Jurkat cell line.

关 键 词：白血病 细胞凋亡 ANNEXIN V BCL-2 PCNA 

分 类 号：R733.71[医药卫生—肿瘤]