介绍一种实体瘤细胞染色体分析的新技术——光谱核型分析  

Application of Spectral Karytyping on Chromosome Analysis of Solid Carcinoma

作　　者：周丽英[1] 钱建新[1] 郭晓葵[1] 戴宏[1] 刘玉龙[1] 周剑影[1] 

机构地区：[1]苏州大学附属第二医院应急中心,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2006年第3期409-411,共3页Suzhou University Journal of Medical Science

摘　　要：目的采用光谱核型分析技术对实体瘤细胞进行染色体分析,为临床对肿瘤的诊断、预后估计和疗效的评价提供重要的判断指标。方法应用多种荧光染料的频谱重叠探针对10例实体瘤细胞进行光谱核型分析。结果我们建立了稳定的光谱核型分析方法,发现肿瘤实体瘤染色体异常呈多倍体且结构异常复杂,包括易位、缺失、扩增、重排、双着丝粒、等臂及标记染色体等。结论光谱核型分析技术可揭示常规核型分析方法难以识别的染色体结构,将成为实体瘤染色体的一种精确、灵敏和可靠的检测手段。Objective Applying Spectral Karytyping （SKY） to analyze chromosome of solid carcinoma, to learn the genesis and growth of solid carcinoma. To provide a significant criterion index for diagnosis, prognosis and evaluation of curative resultant. Methods Adopting frequency spectrum overlapping detecting head of Spectral Karytyping can detect human being all 46 chromosomes by only once hybridization. Results Stable Spectral Karytyping method was set up and extraordinary multiplicity of structure, including metathesis, depletion, amplification, rearrangement, equiarm, markerbody and so on were found by this methad. Conclusion Applying Spectral Karytyping （SKY） to analyze chromosome of solid carcinoma can reveal impalpable chromosome structure. This provides us a precise and sensitive empirical method for analysis chromosome of solid carcinoma in future.

关 键 词：光谱核型分析 荧光原位杂交 实体瘤 染色体 

分 类 号：R730.43[医药卫生—肿瘤]