人巨细胞病毒宫内感染产前诊断方法的研究  

Study on the Prenatal Diagnostic Method for the Intrauterine Human Cy-tomegalovirus Infection

作　　者：邓东锐[1] 闻良珍[1] 陈素华[1] 熬黎明[2] 刘兰清[3] 凌霞珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,湖北武汉430030 [2]上海第二医科大学儿科研究所,上海200092 [3]中国医科大学第二临床学院病毒研究室,辽宁沈阳110001

出　　处：《实用妇产科杂志》2006年第6期370-372,共3页Journal of Practical Obstetrics and Gynecology

基　　金：国家"九五"科技公关课题(项目编号:96-904-06-08)

摘　　要：目的:探索人巨细胞病毒(HCMV)宫内感染产前诊断的最佳方法。方法:应用酶联免疫吸附试验(ELISA)、巢式PCR(N-PCR)和逆转录PCR(RT-PCR)对比分析武汉、上海和沈阳3地3908例孕妇HCMV感染状况及其对妊娠结局的影响。结果:孕妇HCMV IgM检出率为6.24%,与HCMV DNA和晚期mRNA的检测符合率分别为59.03%和56.49%。活动性感染孕妇的宫内传播率(41.58%)明显高于对照组(15.87%)。HCMV晚期mRNA阳性的宫内感染儿流产、早产发生率明显升高。采集绒毛、羊水和脐血进行产前诊断的安全性良好。结论:在应用ELISA法进行初筛的基础上,联合应用N-PCR和RT-PCR技术检测HCMV DNA和晚期mRNA表达,是产前诊断HCMV宫内感染安全而可靠的方法之一。Objective： To explore the best prenatal dagnostic Method for intrauterine human cytomegalovirus （HOMV）Infection.Methods： Enzyme-linked imrnunosorbent assay （ELISA） combined with nasted-polymerase chain reaction（N-PCR） and reverse transcription-PCR （RT-PCR） technique were used to study the state of HCMV infection and the pregnancy outcome in 3908 pregnant women in Wuhan,Shanghai,and Shenyang. Results：The positive rate of HCMV IgM antibody was 6.24% ,and the accordant rate of HCMV DNA and late mRNA with it was 59.03% and 56.49%, respectively. The intrauterine transmission rate of pregnant women with active HCMV infection （41.58%） was significantly higher than that of the control group （15.87%）.The rate of abortion and preterm delivery increased significantly in the intrauterine infective fetus with positive HCMV late mRNA. The prenatal diagnosis collecting from chodonic villus,amniotic fluid and/or cord blood was safe. Conclusions：After screening of HCMV infection during pregnancy by EUSA, the detection of HCMV DNA and late mRNA by N-PCR as well as RT-PCR is a safe and reliable method for prenatal diagnosis of the intrauterine HCMV infection.

关 键 词：人巨细胞病毒 宫内感染 产前诊断 聚合酶链反应 酶联免疫吸附试验 

分 类 号：R914[医药卫生—药物化学]