化学法和机械法从大肠杆菌释放人肿瘤坏死因子  

Releasing of Tumor Necrosis Factor from Recombinant Escherichia Coli by Chemical Permeabilization and High Speed Bead Milling

作　　者：王光虎[1] 冯小黎[1] 修志龙 张志强[1] 苏志国[1] 

机构地区：[1]大连理工大学化工学院生化工程研究所

出　　处：《高校化学工程学报》1996年第3期297-303,共7页Journal of Chemical Engineering of Chinese Universities

基　　金：国家杰出青年科学基金;国家教委跨世纪人才计划基金

摘　　要：对化学法和机械法破碎基因工程大肠杆菌以释放人肿瘤坏死因子的影响因素作了考察。在化学法破菌中研究了破碎时间、试剂浓度、溶液ｐＨ值、加入表面活性剂等因素的影响。在机械法破碎中研究了停留时间、装珠量、搅拌转速、细胞浓度等的影响，对操作条件进行了优化。通过对二者的比较，发现机械法破碎细胞效率高，但产生细小的碎片不利后分离；化学法释放产物有选择性，但操作时间长。The conditions of chemical permeabilization and high speed bead mill in disrupting recombinant E. coli to release TNF were investigated and optimized. Disruption time, guanidine hydrochloride concentration and pH of the solution dominating chemical permeabilization were investigated. Residence time, bead load, agitation speed and biomass concentration that affect high speed bead milling were studied. A comparison was made between the two methods.

关 键 词：基因工程 大肠杆菌 化学法 机械法 肿瘤坏死因子 

分 类 号：TQ464.7[化学工程—制药化工] R394.8[医药卫生—医学遗传学]