环氧合酶-2及其抑制剂对角膜新生血管作用的研究(英文)  

COX-2 and its inhibitor Celecoxib in corneal neovascularization

作　　者：董媛[1] 张明昌[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,中国湖北省武汉市430022

出　　处：《国际眼科杂志》2006年第3期523-526,共4页International Eye Science

摘　　要：目的:观察大鼠角膜新生血管(cornealneovasculariza-tion,CNV)形成过程中环氧合酶-2(COX-2)的表达情况及与CNV的关系,探讨COX-2抑制剂Celecoxib对CNV的抑制作用。方法:利用免疫组织化学方法、RT-PCR法检测碱烧伤后各个时期COX-2和VEGF蛋白、mRNA在角膜各层中的分布,并对其进行半定量。比较实验组和对照组各个时期COX-2和VEGF蛋白和mRNA表达量的差别,并对其进行统计学分析,了解COX-2与VEGF的相关性。结果:①活化的COX-2和VEGF蛋白、mRNA的表达在角膜新生血管的形成中均有一动态变化。②VEGF的表达区域和COX-2表达的部位高度一致。③实验II组和实验Ⅲ组COX-2和VEGF蛋白、mRNA表达量与对照组的差别有显著性(P<0.05),实验I组与对照组的差别无显著性(P>0.05)。实验组和对照组COX-2与VEGF的表达呈正相关。结论:①COX-2在炎症性角膜新生血管形成过程中表达上调,其调控VEGF的表达,在角膜新生血管的形成过程中起着重要作用。②Celecoxib能抑制COX-2的表达,有效的抑制角膜新生血管的形成。AIM： To observe the expression of COX-2 in rat corneal neovascularization （CNV） and its relationship to CNV, and to explore the inhibition of Celecoxib, a COX-2 inhibitor, to CNV. METHODS： The distribution and quantification of COX-2and VEGF was detected by immunohistochemistry. Expression of COX-2 and VEGF mRNA was quantified by RT-PCR.The difference in protein and mRNA expressions of COX-2and VEGF was analyzed to find the correlation between them. RESULTS： Expression of activated COX-2 and VEGF protein and mRNA in CNV had a dynamic change. VEGF and COX-2co-localized. Compared with the control group, expression of both protein, mRNA of COX-2 and VEGF in experimental group Ⅱ and Ⅲ had significant difference （P〈0.05）, indicating the correlation between COX-2 and VEGF, while that in experimental group I had no statistical difference （P〉0.05）. CONCLUSION： COX-2 expression was up-regulated in inflammatory CNV. COX-2 modulates the expression of VEGF,playing a very important role in CNV. Celecoxib inhibit COX-2expression so as to hold back the CNV.

关 键 词：角膜新生血管 血管内皮生长因子 环氧合酶-2 CELECOXIB 

分 类 号：R772[医药卫生—眼科]