2000—2004年浙江省食品中产单核李斯特菌污染状况调查  

Investigation on Listeria monocytogenes contamination in food of Zhejiang Province during 2000- 2004

作　　者：梅玲玲[1] 程苏云[1] 朱敏[1] 王赞信[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310009

出　　处：《中国卫生检验杂志》2006年第7期784-785,815,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：掌握浙江省食品中产单核李斯特菌污染状况。以及内化素基因（Inl）和李氏溶血素O（Hly）两种致病基因携带情况。方法：按GB4789．30方法分离单增生李斯特菌，采用PCR技术检测李斯特菌的两种致病基因。结果：2000～2004年从2282份食品中共分离产单核李斯特菌163株，总污染率为7．14％（163／2282）。生肉类、熟肉及水产品的污染率分别为12．80％、7．67％和2．24％；114份生食蔬菜中1份标本分离到产单核李斯特菌；285份乳及乳制品、59份冰激凌均未分离到产单核李斯特菌；163株产单核李斯特菌有155株同时检测到内化素基因和李氏溶血素O基因。结论：我省存在发生李斯特菌食物中毒的潜在危险。Objective：To find out the practical situation of L. monocytogenes contamination in food of Zhejiang Province and detect carrying rate of the two pathogenic genes Inl and Hly. Methods：L. monocytogenes strain was isolated according to GB - 4789. 30 and its two pathogenic genes were detected by PCR. Results： An examination on 2 282 samples of food from Zhejiang Province for the detection of L. monocytogenes was carried out in 2000 - 2004, 163 cases of Lm were found, that means a total contamination rate of 7. 14%. Its contamination rates detected in raw meat, cooked meat and fishery products were 12. 80%, 7.67% and 2. 24% respectively. L. monocytogenes strain was not detected in the 285 milk or dairy products samples and 59 ice cream samples. Among the total 169 cases, 155 of them carried both Listeriolysin O and Internalin gene. Conclusion ：There is the potential threat of food poisoning caused by L. monocytogenes in Zhejiang Province.

关 键 词：浙江省 食品 产单核李斯特菌 内化素 李氏溶血素O 

分 类 号：R155.5[医药卫生—营养与食品卫生学]