婴幼儿配方奶粉中氯霉素残留的酶联免疫分析法  

DETERMINATION OF CHORAMPHENICOL RESIDUES IN INFANT FORMULA POWDERS BY ENZYME IMMUNOASSAY METHOD

作　　者：陈燕勤[1] 庄逸林[1] 陈小雪[1] 张胜举[1] 

机构地区：[1]汕头出入境检验检疫局,广东汕头515041

出　　处：《检验检疫科学》2006年第3期11-12,共2页Inspection and Quarantine Science

摘　　要：〔目的〕建立测定婴幼儿配方奶粉中氯霉素残留的酶联免疫分析法。〔方法〕用乙酸乙酯对样品中的氯霉素残留进行提取,用酶联免疫试剂盒进行检测。〔结果〕方法检测低限为:0.075μg/kg;在0.1μg/kg和0.3μg/kg两个添加水平,样品加标回收率:分别为85%～120%和91%～119%;重复性试验:CV%分别为6.35%和5.31%;重现性试验:CV%分别为11.88%和12.92%。〔结论〕本方法灵敏度、准确度和精密度均符合残留分析质量控制的要求,适用于婴幼儿配方奶粉中氯霉素残留的快速检测。An enzyme immunoassay method for the determination of choramphemcol residues m lntant formula powoers is presented. The choramphenicol residues in samples were extracted with ethyl acetate, and then detected using an enzyme immunoassay kit. The limit of detection （LOD） was 0.075μg/kg; On the spiked levels of 0.1μg/kg and 0.3μg/kg, the recoveries were 85%-120% and 91%-119%,respectively,with the CV% for the repeatability of 6.35%and5.31% and the CV% for the reproducibility of 11.88% and 12.92%. The sensitivity, accuracy and precision of the method were in accordance with the requirements for quality control of residues analysis. It is suitable for the rapid detection of choramphenicol residues in infant formula powders.

关 键 词：婴幼儿配方奶粉 氯霉素 残留量 酶联免疫分析 

分 类 号：S859.84[农业科学—临床兽医学]