棒束孢霉胞外多糖的纯化  

PURIFICATION OF THE EXTRACELLULAR POLYSACCHARIDES CULTURED FROM ISARIA SP.

作　　者：褚西宁[1] 程巧梅[1] 袁静明[1] 

机构地区：[1]山西大学生命科学系

出　　处：《山西大学学报（自然科学版）》1996年第3期315-318,共4页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省自然科学基金资助项目

摘　　要：棒束孢霉发酵上清液经减压浓缩、醇析、蛋白酶法与Ｓｅｖａｇ法结合脱蛋白、逆向流水透析和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０柱层析等步骤，获得了胞外纯多糖。用ＴＬＣ、ＧＣ及ＩＲ分析表明，该多糖链由Ｄ－甘露醇和Ｄ－半乳糖组成。A strain named Isaria sp. was separated and purified from Cordyceps sinensis.The extracellular polysaccharide from 7 days culture of the strain were purified by some steps,eg.concentration in vacum,preciptation with alcohol,deprotrinization with both subtilism and sevag procedure as well as chromatography on DEAE-Sephadex A-50 column.The results from TLC,GC and IR analysis showed that the polysaccharide consisted of two compounds:D-galactose and D-mannose only.

关 键 词：棒束孢霉 真菌多糖 纯化 冬虫夏草 

分 类 号：R284.2[医药卫生—中药学]