咔唑比色法测定豆腐柴叶果胶含量的研究  

Determination of premna microphylla pectin content by carbazole spectrophotometric method

作　　者：徐汶[1,2] 王光辉[1] 王存文[2] 陈亚琴[2] 王为国[2] 张俊峰[2] 

机构地区：[1]武汉科技大学化工与资源环境学院,湖北武汉430081 [2]武汉化工学院化工与制药学院

出　　处：《食品与机械》2006年第3期133-135,共3页Food and Machinery

基　　金：湖北省教育厅科学研究计划2001年重大项目(2001Z20005)

摘　　要：介绍了采用咔唑硫酸分光光度法测定豆腐柴叶果胶粉果胶含量的方法,对其测定条件作了详细的试验,获得了较佳的测定条件浓硫酸用量为5.0mL,水解温度为75℃,水解时间为15min,0.15%咔唑无水乙醇溶液用量为0.2mL,显色温度为25℃左右,显色时间为2h。用于实际样品的测定时,结果较为满意。A method is described for the determination of pectin content in Premna Microphylla pectin by carbazole and sulphuric acid spectrophotometric method, experiment conditions are investigated in detail, and the optimum conditions are found as follows： sulphuric acid 5.0 ml, hydrolytic temperature 75 ℃, hydrolytic time 15 min, 0.15% carbazole absolute ethanol＇s solution 0.2 mL, reaction temperature 25 ℃, reaction time 2 h, the method has been applied successfully to actual samples and satifactory results are obtained.

关 键 词：果胶 半乳糖醛酸 光度测定法 咔唑 硫酸 

分 类 号：TS201[轻工技术与工程—食品科学]