睡眠剥夺及睡眠恢复后大鼠视交叉上核星形胶质细胞反应及其与神经元的关系  

Response of astrocytes to sleep deprivation and sleep recovery and its relationship with neurons at rat's suprachiasmaticus nucleus

作　　者：惠雪枫[1] 宿长军[1] 李柱一[1] 张润宁 

机构地区：[1]第四军医大学唐都医院神经内科 [2]陕西省第二纺织医院神经内科,陕西咸阳712000

出　　处：《医学研究生学报》2006年第7期592-595,共4页Journal of Medical Postgraduates

基　　金：第四军医大学军事医学研究项目基金资助(批准号:05XJM001)

摘　　要：目的:探索睡眠剥夺及睡眠恢复后大鼠视交叉上核(SCN)星形胶质细胞的反应及其与神经元的关系。方法:采用水环境小平台法建立大鼠睡眠剥夺模型,12只大鼠随机分为睡眠剥夺(SD)24 h组,睡眠剥夺24 h后恢复睡眠(SR)3 h组,大平台对照组(TC)和正常饲养组(NC),每组3只。用免疫组化方法检测胶原纤维酸性蛋白(GFAP)和Fos神经元在SCN的表达变化。结果:睡眠剥夺后GFAP和Fos在SCN表达明显增强,睡眠恢复后两者表达都降低,变化趋势基本一致。结论:星形胶质细胞可能与神经元共同参与睡眠的调节。Objective：To investigate the response of the astrocytes to sleep deprivation and sleep recovery at rat＇s SCN, as well as its relationship with neurons. Methods：12 Sprague-Damley rats were divided into four groups at random. Two groups were deprived of sleep by housing them on the small platforms over water for 24h and then one group recovered sleep for 3h. Controls were housed in the normal cage and on the large platforms over water. There were 3 rats in each group. The effects of sleep deprivation and sleep recovery on the expression of glial fibrillary acidic protein（GFAP） and Fos protein were investigated by immunohistochemistry. Results ：The expressions of GFAP and Fos were increased obviously at SCN after sleep deprivation, where both of them were depressed after sleep recovery and the tendencies of these changes were almost at equal pace. Conclusion ： It is possible that the astrocytes regulate sleep together with neurons.

关 键 词：睡眠剥夺 视交叉上核 星形胶质细胞 FOS 大鼠 

分 类 号：R338[医药卫生—人体生理学]