Survivin反义寡核苷酸抑制肝癌移植瘤生长的作用  

Inhibitory effect of Survivin antisense oligonucleotide on growth of human hepatic carcinoma transplanted subcutaneously

作　　者：高鹏[1] 张刚庆[2] 颜政[3] 方驰华[3] 

机构地区：[1]广东省第二人民医院普外科,广东省广州市510317 [2]中山大学附属第三医院,广东省第二人民医院普外科博士后科研基地,广东省广州市510317 [3]南方医科大学附属珠江医院肝胆外科,广东省广州市510282

出　　处：《世界华人消化杂志》2006年第16期1627-1630,共4页World Chinese Journal of Digestology

摘　　要：目的:检测Survivin反义寡核苷酸(ASODN)在人肝癌耐药细胞株裸鼠皮下移植瘤中的表达情况．方法:将30只裸鼠建立人肝癌耐药细胞系 SMMC-7721／ADM皮下移植瘤模型,随机分成6组:空白对照组(A)、脂质体转染对照组 (B)、正义链对照组(C)、200(D)、400(E)和 600 μg/L(F)反义链组(ASODN组),用不同的转染液注射后2,4,8,12,16,20 d,用逆转录聚合酶链反应技术(RT-PCR)和Western blot蛋白免疫印迹法检测治疗后各组肿瘤组织中Survivin mRNA和蛋白表达的变化．结果:注射后20 d,ASODN组肿瘤细胞生长明显抑制,空白对照组、脂质体转染对照组和正义链对照组裸鼠的mRNA和蛋白表达无明显差异,而ASODN组mRNA和蛋白表达随着时间和浓度的增加,Survivin表达减弱,E,F组与其余个组(A,B,C,D)相比有显著差异(mRNA: 0．33±0．04,0．28±0．03 vs 0．82±0．02,0．78± 0．05,0．72±0．04,0．57±0．03,P<0．05;蛋白: 34．9±3．89,21．2±3．65 vs 72．14±6．53,69．31 ±5.34,68．29±4．98,53．8±5．23,P<0．05)．结论:Survivin反义寡核苷酸能够下调 Survivin mRNA和蛋白的表达,抑制裸鼠皮下移植瘤的生长．AIM： To explore the effects of liposomes Survivin antisense oligonucleotides （ASODN） on the growth of human hepatic carcinoma transplanted subcutaneously in nude mice. METHODS： Human hepatic carcinoma transplanted subcutaneously was established in nude mice. Then the animals were randomly divided into 6 groups： control group （A）, liposome group （B）, sense oligonucleotide （SODN） group （C）, 200 （D）, 400 （E） and 600μg/L （F） ASODN group. After treatment with the corresponding methods for 2, 4, 8,12, 16, 20 d, the expression of Survivin rnRNA and protein detected by reverse transcription-polymerase chain reaction andWestern blot, respectively. RESULTS： The growth of the tumors was significantly inhibited in all ASODN groups as compared with that in the control, liposome and SODN group after treatment for 20 d. The number of apoptotic cells in ASODN group increased and Survivin expression became weakened. The expression of Survivin was significantly lower in group E and F than those in group A, B, C, and D （mRNA： 0.33±0.04, 0.28±0.03 vs 0.82±0.02, 0.78±0.05, 0.72±0.04, 0.57± 0.03, P〈0.05; protein： 34.9±3.89, 21.2±3.65 vs 72.14±6.53, 69.31 ±5.34, 68.29±4.98, 53.8±5.23, P〈0.05）. CONCLUSION： Survivin ASODN can inhibit the growth of human hepatic carcinoma in nude mice by inducing cell apoptosis and decreasing Survivin expression.

关 键 词：反义寡核苷酸 肝癌 Survivin 疾病模型 裸鼠 

分 类 号：R735.7[医药卫生—肿瘤]