水牛SRY基因的克隆及序列分析  

Cloning and Characterization of Swamp Buffalo SRY Gene

作　　者：付强[1] 张明[1] 秦文松[1] 郑海英[1] 卢克焕[1] 

机构地区：[1]广西亚热带生物资源保护利用重点实验室

出　　处：《中国生物工程杂志》2006年第7期69-73,共5页China Biotechnology

基　　金：省部共建国家重点实验室培育基地"广西亚热带生物资源保护利用重点实验室"开放课题基金资助项目(No.S80403)

摘　　要：根据荷斯坦牛SRY基因设计一对引物,采用聚合酶链式反应(PCR)技术,以中国沼泽型水牛(Swamp Buffalo)基因组DNA为模板,扩增得到SRY(Sex deterimation region of Y chromosome)全序列约2005bp,其中1-504bp为5’启动子区,1196-2005bp为3’侧翼序列,在505-1195bp为SRY的外显子,编码229个氨基酸。在SRY HMG-box区域设计探针,用地高辛标记后分别与雄性、雌性水牛基因组DNA进行Southern杂交,结果显示该段序列只在雄性DNA样本中有杂交信号,证明SRY基因为雄性特异。BLAST比对结果显示与牛属动物SRY基因的同源性为96％,其中SRY基因HMG box区域同源性高达99％,说明SRY基因具有高度的进化保守性。The SRY gene from buffalo （Bubalus bubalis） genome was amplified by the polymerase chain reaction （PCR） with primers based on the sequence of Hostein SRY gene. The amplified fragment was 2005 bp include 5UTR （1 ～ 504bp） and 3＇ UTR （1196～ 2005bp）. And the amplified fragment was cloned and sequenced. Sequence analysis showed that the coding region of SRY gene （505 ～ 1195bp） from buffalo was highly homologous with those of other bovine counterpart genes （96% homology）, especially in the HMG box region （99% homology）. It was found that there were only signal on male buffalo genome on Southern blot,which indicate SRY gene are highly conservative on evolves.

关 键 词：水牛 SRY基因 Southem杂交 

分 类 号：Q785[生物学—分子生物学]