农杆菌介导的马铃薯茎段遗传转化体系优化研究  

An Efficient Transformation System of Potato Mediated by Agrobacterium Tumefaciens

作　　者：贾笑英[1] 路平[1] 王蒂[1] 

机构地区：[1]甘肃农业大学农学院,兰州730070

出　　处：《中国农学通报》2006年第7期79-81,共3页Chinese Agricultural Science Bulletin

基　　金：国家863计划资助项目"用生物技术培育抗旱和抗低温马铃薯品种研究"(2004AA241132);甘肃省科技攻关项目"黄土高原半干旱地区水肥高效利用加工型马铃薯新品种选育"(2GS054-A41-005-01)。

摘　　要：以甘肃主栽品种的茎段为外植体,研究了不同转化条件(菌液浓度、感染时间、预培养及共培养时间)对转化效率的影响,以及选择压和培养基对抗性出芽的影响,初步建立了马铃薯品种“陇薯3号”和“台湾红皮”的遗传转化体系并获得了转化植株。卡那霉素生根筛选和PCR鉴定结果表明,再生的转化植株假阳性率比较高,要获得预期的转基因植株群体需加大遗传转化工作中转基因植株的数量。Stem segments from potato cuhivars of Gansu were used as receptor materials to study the effects of different cuhivars, explants and different transformation conditions （the concentration of A. tumefaciens, infecting period, length of precuhure and cocuhure） on transformation efficiencies, and the effects of kannamycin concentration and media on resistant callus shooting. Transformation systems for two potato cultivars ＂Longshu No.3＂ and ＂Cardinal＂ were preliminarily set up and the transgenic plants were also obtained. Rooting selection on kannamycin and PCR identification demonstrated that the false positive efficiency of transformation is higher among the putative transgenic plants, and suggested that the desired transgenic plants could be obtained from a large number of transgenic plants in potato ransformation.

关 键 词：马铃薯 根癌农杆菌 茎段 遗传转化 

分 类 号：S532[农业科学—作物学]