反相高效液相色谱法测定酵母RNA降解的5'-核苷酸  

DETERMINATION OF 5'-NUCLEOTIDES DEGRADED FROM THE RNA OF YEASTS BY RP-HPLC

作　　者：王晓丽[1] 郑飞云[1] 李永仙[1] 李崎[1] 顾国贤[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《食品研究与开发》2006年第6期130-131,共2页Food Research and Development

摘　　要：建立了一种利用反相高效液相色谱法同时测定啤酒废酵母提取的核糖核酸(RNA)降解生成的4种核苷酸(5'-AMP、5'-CMP、5'-GMP、5'-UMP)的方法,应用反相μBondapakC18柱,水:甲醇:冰醋酸:四丁基氢氧化铵(TBAOH,10%)=883.5:100:15:1.5,检测波长254nm,4种核苷酸能一次性分离,回收率均达到96%以上,标准偏差分别为2.74%、0.98%、1.65%和1.23%。Four nucleotides （5＇-AMP,5＇-CMP,5＇-GMP,5＇-UMP） degraded from the RNA of yeasts were separated by reversed phase high performance liquid chromatography at the same time. It could be detected by using μ Bondapak C18 column at 254 nm by UV detector with water： methyl alcohol ： acetic acid ： tetrabutylammonium hydroxide （894.5：100： 5：0.5） as mobile phase. Recovery of four nucleotides was higher than 96 %. The accuracy of the four nucleotides was 2.74 %, 0.98 %, 1.65 % and 1.23 %, respectively.

关 键 词：核苷酸 反相高效液相色谱 核糖核酸 啤酒废酵母 

分 类 号：Q522-33[生物学—生物化学]