血管紧张素转化酶抑制剂体外活性检测方法的改进及应用  

Validation and application of an angiotensin-converting enzyme inhibition analysis method

作　　者：蒋菁莉[1] 任发政[1] 诸晓强[2] 

机构地区：[1]中国农业大学食品科学与营养工程学院教育部北京市功能乳品实验室,北京100083 [2]黑龙江省乳品工业技术开发中心,黑龙江哈尔滨150086

出　　处：《中国乳品工业》2006年第7期9-12,33,共5页China Dairy Industry

基　　金："十五"奶业专项基金资助项目(2002BA518A-05)

摘　　要：通过测定不同缓冲体系、氯离子浓度、酶底物比等对血管紧张素转化酶(ACE)活力测定反应体系的影响,建立了适用于食源性ACE抑制剂体外活性检测的分析方法,并分析了该方法的可靠性。测定方法条件为:浓度为200mmol/LNaCl的Tris-HCl缓冲体系,反应温度37℃,检测342nm波长下吸光值在7min内的连续变化;用已知的ACE抑制剂Captopril对方法进行检验,并将其应用于乳蛋白质不同酶水解产物ACE抑制活性的测定。The effect of buffer, the ratio of enzyme and substrate and the concentration of chloride ions on the activity of angiotensin- con verting enzyme （ACE） were detected, and establish an analysis method for screening of food-derived ACE inhibition, the reliability of the method was assay. The parameter of the method is： 75 mmol/L Tris-HCl buffer with 200 mmol/L NaCI, pH 8.3, the temperature of reaction is 37 ℃, the absorption decrease at 340 nm during a period of 7 min was detected. The ACE inhibition assay was further validated by captopril, the ACE inhibitory activity of milk protein and the hydrolysate of casein was detected.

关 键 词：血管紧张素转化酶 血管紧张素转化酶抑制剂 体外活性检测 多肽 

分 类 号：TQ516[化学工程]