黑麂粪便DNA提取及其PCR检测  

Black Muntjac Faecal DNA Extraction Techniques in PCR Reaction

作　　者：程宏毅[1] 鲍毅新[1] 郑荣泉[1] 陈良[1] 葛宝明[1] 

机构地区：[1]浙江师范大学生态研究所,金华321004

出　　处：《生态科学》2006年第2期158-161,共4页Ecological Science

基　　金：国家自然科学基金资助项目(30370219)

摘　　要：采集了黑麂(Muntiacuscrinifrons)的新鲜粪便以及在野外自然条件下保存较长时间的粪便样品,晾干后带回实验室,提取其DNA;同时提取黑麂肌肉、皮张样品的DNA,用以对比粪便样品的提取效果。电泳检测结果显示,此方法使用实验室中常用的分子生物学试剂,可以从黑麂粪便样品中抽提到高质量的粪便DNA并克服分子粪便学研究中常见的PCR反应抑制物的影响。为其它濒危鹿科动物的非损伤性取样提供了的新途径,为其遗传结构、遗传多样性现状等研究提供了更加广阔的取材空间。The fresh faecal and dated faecal of black muntjac （Muntiacus crinifrons） were collected, and their DNA were extracted. For comparing the extraction efficiency, the muscle tissue and skins samples of black muntjac were extracted. The electrophoresis results showed that this method can efficiently extract faecal DNA from black muntjac＇s faecal samples. This method overcame previous barriers to molecular scatology, new approach is developed for noninvasive samping of black muntjac and chinese sika deer. Noninvasive samping can expand the extension of sampling for genetic population structure and genetic diversity of highly endangered cervid.

关 键 词：黑麂 粪便DNA DNA抽提 PCR检测 非损伤性取样 

分 类 号：Q953[生物学—动物学] Q959.843