Expression of angiopoietins, Tie2 and vascular endothelial growth factor in angiogenesis and progression of hepatocellular carcinoma  

作　　者：Zhong-Lin Zhang Zhi-Su Liu Quan Sun 

机构地区：[1]Department of General Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China

出　　处：《World Journal of Gastroenterology》2006年第26期4241-4245,共5页世界胃肠病学杂志（英文版）

摘　　要：AIM： TO investigate the significance of angiopoietins, Tie2 and vascular endothelial growth factor （VEGF） expression in the angiogenesis and progress of hepatocellular carcinoma （HCC）. METHODS： Fresh surgically resected specimens of HCC and noncancerous liver （NCL） tissue from 38 patients with HCC were obtained, and expression of angiopoietin-1 （Ang-1）, angiopoietin-2 （Ang-2）, Tie2, and VEGF messenger RNA （mRNA） was examined by real-time quantitative reverse transcription-polymerase chain reaction （RT-PCR）. Expression pattern of each gene in HCC and NCL tissue specimens was compared and the potential role and interaction in angiogenesis of HCC were analyzed. Genes＇ expression level and its relationship with tumor＇s clinicopathological parameters were also investigated. Immunohistochemical staining of CD34 was performed to determine the microvessel density （MVD） and Ang-2/Ang-1 ratio was calculated. Relationships between Ang-2/Ang-1 ratio, VEGF and MVD and clinicopathological features were also tested so as to evaluate their significance in the progression of HCC. RESULTS： Ang-2 and VEGF mRNAs in HCC were significantly higher than those in NCL tissue （P 〈 0.05）, whereas the Ang-1 and Tie2 mRNAs showed no statistical significance （P 〉 0.05）, though slightly lower level of Ang-1 mRNA in HCC was observed. Ang-2/ Ang-1 ratio and VEGF were both positively correlated to MVD. The Ang-2/Ang-1 ratio, Ang-2 and VEGF were all associated with tumor＇s clinicopathological parameters （P 〈 0.05） except for histological grades （P 〉 0.05）. Ang-1 and Tie2 levels in different clinicopathological groups were not significantly different （P 〉 0.05）. CONCLUSION： Dominant Ang-2 expression against Ang-1 through Tie2 receptor in the presence of VEGF plays a critical role in initiating early neovascularization and transformation of noncancerous liver to hepatocellular carcinoma. Its consequently constant operation in formed HCC induces further angiogenesis and progre瞄准：调查 angiopoietins， Tie2 和脉管的内皮的意义在肝细胞癌(HCC ) 的血管生成和进步的生长因素(VEGF ) 表示。方法：新鲜通过手术 HCC 和非癌的肝( NCL )的 resected 标本从有 HCC 的 38 个病人的织物被获得，并且 angiopoietin-1 ( Ang-1 )的表示， angiopoietin-2 ( Ang-2 )， Tie2 ，并且 VEGF 送信人 RNA ( mRNA )被即时量的反向的抄写聚合酶链反应( RT-PCR )检验。在 HCC 和 NCL 织物标本的每基因的表示模式被比较，在 HCC 的血管生成的潜在的角色和相互作用被分析。基因有肿瘤的 clinicopathological 参数的表达式水平和它的关系也被调查。CD34 染色的 Immunohistochemical 被执行决定微容器密度(MVD ) 和 Ang-2/Ang-1 比率是计算的。在 Ang-2/Ang-1 比率， VEGF 和 MVD 和 clinicopathological 特征之间的关系也被测试以便在 HCC 的前进评估他们的意义。结果：在 HCC 的 Ang-2 和 VEGF mRNAs 比在 NCL 织物的那些显著地高(P < 0.05 ) 而 Ang-1 和 Tie2 mRNAs 没显示出统计意义(P > 0.05 ) ，不过在 HCC 的 Ang-1 mRNA 稍微低级被观察。Ang-2/Ang-1 比率和 VEGF 断然两个都被相关到 MVD。Ang-2/ Ang-1 比率， Ang-2 和 VEGF 都与肿瘤的 clinicopathological 参数被联系(P < 0.05 ) 除了组织学的等级(P > 0.05 ) 。在不同 clinicopathological 组的 Ang-1 和 Tie2 层次不是显著地不同的(P > 0.05 ) 。结论：对通过 Tie2 受体的 Ang-1 的主导的 Ang-2 表示在开始非癌的肝的早 neovascularization 和转变到肝细胞癌面对 VEGF 起一个关键作用。它处于良好的竟技状态 HCC 的因而经常的操作导致 HCC 的进一步的血管生成和前进。

关 键 词：Hepatocellular carcinoma Vascular endothelial growth factor ANGIOPOIETIN TIE2 ANGIOGENESIS Neovascularization 

分 类 号：R735.7[医药卫生—肿瘤]