羧甲基壳聚糖对人高转移肺癌细胞—95-D增殖能力和体外转移能力的抑制作用  

Inhibitory effects of carboxymethyl-chitosan on proliferation and in vitro metastatic ability in human highly metastatic lung carcinoma 95-D Cells

作　　者：杨靖亚[1] 刘建文[2] 刘承初[1] 陈必文[1] 

机构地区：[1]上海水产大学食品学院,上海200090 [2]华东理工大学药学院,上海200237

出　　处：《中国临床药理学与治疗学》2006年第6期640-643,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：上海水产大学博士基金项目(№04-166);上海市重点学科建设项目(№T1102)

摘　　要：目的：评价羧甲基壳聚糖对人高转移肺癌细胞-95-D增殖能力和体外转移能力的抑制作用。方法：用软琼脂克隆形成实验、创伤实验、黏附实验和transwell小室实验来评价羧甲基壳聚糖对95．D细胞的影响。结果：羧甲基壳聚糖浓度在0～150μg·ml^-1范围内，剂量依赖性地抑制95-D细胞的增殖能力、移动能力、浸润能力、黏附能力和克隆形成能力。结论：羧甲基壳聚糖能有效抑制95-D细胞的增殖能力和体外转移能力。AIM： To study the inhibitory effects of carboxymethyl-chitosan on proliferation and metastatic ability in human highly metastatic lung carcinoma 95-D Cells. METHODS：The inhibitory effect of earboxymethyl-chitosan on the migration of 95-D cells was tested by cell migration assay. Cell invasion was analyzed by the matrigel invasion assay. Soft agar colony formation assay and adhesion assay were also used to evaluate the suppression of carboxymethyl-chitosan on 95-D cells. RESULTS： Carboxymethyl-chitosan dose-dependently inhibited the soft agar colony formation, migration, invasion, adhesion and proliferation of 95-D cells. CONCLUSION： Carboxymethyl-chitosan can significantly repress the proliferation and in vitro metastasis of 95-D cells.

关 键 词：羧甲基壳聚糖 转移 肺癌 

分 类 号：R73[医药卫生—肿瘤] R965.1[医药卫生—临床医学]