全长人lrp基因及其截短和定点突变体真核表达载体的构建  

Construction of Eukaryotic Expression Vector Inserted Human lrp Gene, Its Truncate and Mutate Sequences

作　　者：宋庆贺[1] 陈苏民[1] 陈南春[1] 代忠明[1] 侯立朝[2] 于新平[2] 

机构地区：[1]第四军医大学生物化学与分子生物学教研室 [2]西京医院麻醉科,西安710032

出　　处：《科学技术与工程》2006年第14期2016-2018,2023,共4页Science Technology and Engineering

基　　金：国家自然科学基金(30471675)资助

摘　　要：为了对脂多糖应答基因(lrp)的功能进行深入的研究,实验用PCR及双PCR法扩增出lrp基因的截短体(lrpΔC)和突变体(lrpm)序列,测序正确后将正常lrp基因及其截短体和突变体序列均连入真核表达载体pcDNA3.1(+),构建重组质粒pcDNA3.1(+)-lrp、pcDNA3.1(+)-lrpΔC和pcDNA3.1(+)-lrpm。DNA测序结果显示,PCR反应成功得到了lrp基因的截短体和突变体序列;重组质粒酶切鉴定结果显示:人lrp基因及其截短体、突变体成功连入了真核表达载体pcDNA3.1(+)。To research the lrp gene function further, truncate and mutate sequences of lrp gene were amplified by PCR. Then wild lrp gene, its truncate and mutate sequences were all inserted eukaryotic expression vector pcDNA3.1 （＋） to construction recombinant plasmid pcDNA3.1 （＋）-lrp, DNA3.1 （＋）-lrp△C and pcDNA3.1 （＋）-/rpm. DNA sequencing shows that truncate and mutate sequences of lrp gene were got successfully by PCR. And the results of enzyme digesting analysis of recombinant plasmids show that the wild lrp gene, its truncate and mutate sequences were all inserted eukaryotic expression vector pcDNA3.1 （＋）.

关 键 词：人脂多糖应答基因 突变 真核表达载体 

分 类 号：Q257[生物学—细胞生物学] Q78