辽宁汉族人群HLA-B等位基因多态性的分布  

Distribution of HLA-B allele polymorphism of 8962 Han population in Liaoning of China

作　　者：陈阳 李剑平 

机构地区：[1]辽宁省血液中心输血医学研究所,沈阳110044

出　　处：《中华医学遗传学杂志》2006年第4期461-462,共2页Chinese Journal of Medical Genetics

摘　　要：目的调查辽宁汉族人群HLA-B等位基因的遗传多态性。方法用聚合酶链反应-序列特异性引物方法对辽宁8962名健康无关汉族人进行HLA-B等位基因分型,计算HLA-B等位基因频率并与不同人群HLA-B等位基因的多态性进行比较。结果共检出HLA-B等位基因34种,其中B*15(14.42%)、B*40(14·33%)和B*13(11.99%)基因频率分布较高,B*82、B*83等位基因未检出;HLA-B座位特异性49种。该人群与南北方汉族人群、日本人、黑人和白人分别进行χ2检验差异有统计学意义,χ2值分别为1584.799、72.145、1393.339、7406.288和5311.947。结论辽宁汉族人群HLA-B基因多态性分布有其自身特点,它的遗传特征不同于既往的南、北方汉族。Objective To investigate the distribution of genetic polymorphism of HLA-B locus in Liaoning Han population. Methods The technique of polymerase chain reaction-sequence specific primers （PCR-SSP） was adopted in genotyping a sample of 8962 unrelated healthy individuals collected from a Chinese Hart population in Liaoning area. The allele frequency of HLA-B was calculated and was compared with the results from other population. Results Thirty-four HLA-B alleles were detected. The more common HLA-B allele included B＊ 15（14.42%）,B＊ 40（14.33%） and B＊ 13（ 11.99% ）. The B ＊ 82 and B ＊ 83 were absent. Forty-nine HLA-B antigens were detected. Conclusion The HLA- B allele polymorphism distribution of Liaoning Hart population exhibits its own characteristic and the difference from old south and noith Han population in China.

关 键 词：聚合酶链反应-序列特异性引物 HIA-B等位基因 遗传多态性 等位基因频率 

分 类 号：R394[医药卫生—医学遗传学]