吡格列酮对高脂饮食兔主动脉CD40L表达的影响  

The Effects of Pioglitazone on the Expression of CD40L in High-Cholesterol Diet Rabbit Aorta

作　　者：楚罗湘[1] 姜德谦[1] 刘照云[1] 

机构地区：[1]中南大学湘雅二医院心内科,湖南省长沙市410011

出　　处：《中国动脉硬化杂志》2006年第6期491-494,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨吡格列酮对高脂饮食兔主动脉CD40L表达的影响。方法设立正常饮食、高脂饮食及高脂饮食加吡格列酮干预三组,比较主动脉病理形态学改变、血脂变化,采用免疫组织化学检测兔主动脉CD40L的表达,采用逆转录聚合酶链反应检测兔主动脉凝血酶样氧化型低密度脂蛋白受体1 mRNA的表达。结果吡格列酮能减轻高脂饮食所致的内膜增厚和平滑肌细胞增殖,吡格列酮还能明显升高高密度脂蛋白胆固醇水平。高脂饮食刺激兔主动脉CD40L的表达,吡格列酮能显著减轻这种作用。高脂饮食能上调兔主动脉表达凝血酶样氧化型低密度脂蛋白受体1 mRNA的表达,而吡格列酮能显著抑制这种作用。结论吡格列酮能减轻高脂饮食兔主动脉CD40L的表达,其作用可能与抑制凝血酶样氧化型低密度脂蛋白受体1 mRNA表达有关,提示吡格列酮可通过抑制高脂血症患者炎症信号通路而达到抗动脉硬化的作用。Aim To test the hypothesis that Pioglitazone decrease CD40L expression in hypercholesterolemia rabbit aorta. Methods 15 male healthy New Zealand rabbits were randomly divided into three groups： control group （ n = 5 ）was fed with normal diet for eight weeks; high cholesterol group was fed with high-cholesterol diet for eight weeks; Pioglitazone treatment group （n = 5）was fed with high-cholesterol diet for eight weeks supplemented with Pioglitazone [3 mg/（kg·d）] from the fourth week of cholesterol diet. CD40L was detected by immunohistochemistry, lectin-like oxidized low density lipoprotein receptor-1 （LOX-1） mRNA was evaluated by reverse transcription-polymerase chain reaction （RT-PCR）. Results Aortic intimal hyperplasia and smooth muscle cells proliferation in rabbits fed with high-cholesterol diet were decreased. Pioglitazone could improve high density lipoprotein and decrease the expression of CD40L and LOX-1 mRNA in hypercholesterolemia rabbits aorta. Conclusions Pioglitazone can inhibit the upregulation of LOX-1 in hypereholesterolemia rabbits aorta and decrease CD40L expression. These observation provide novel insight into a potential novel antiinflammatory and antiatherosclerosis pathway of thiazolidinediones.

关 键 词：病理学与病理生理学 吡格列酮的抗炎及抗动脉粥样硬化作用 免疫组织化学 吡格列酮 高脂血症 CD40L 凝血酶样氧化型低密度脂蛋白受体1 

分 类 号：R363[医药卫生—病理学]