同型半胱氨酸对大鼠血管平滑肌细胞基质金属蛋白酶2表达的影响  

Effects of Homocysteine on the Expression of Matrix Metalloproteinase-2 in Rat Vascular Smooth Muscle Cells

作　　者：邢杨波[1] 郭航远[1] 马孝泉[1] 何红[2] 

机构地区：[1]绍兴市人民医院心内科,浙江省绍兴市312000 [2]浙江大学医学院附属邵逸夫医院心内科,浙江省杭州市310016

出　　处：《中国动脉硬化杂志》2006年第6期508-510,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的 观察同型半胱氨酸对大鼠血管平滑肌细胞基质金属蛋白酶2表达的影响，探讨其致动脉粥样硬化可能的机制。方法 体外培养大鼠主动脉平滑肌细胞，加入不同浓度的同型半胱氨酸分别作用24、48和72h，采用免疫印迹法和明胶酶谱分析法检测细胞培养基中基质金属蛋白酶2的表达。结果 同型半胱氨酸浓度在0．5～1．0mmol／L时促进血管平滑肌细胞基质金属蛋白酶2的表达；浓度在5．0mmol／L以上时抑制血管平滑肌细胞基质金属蛋白酶2的表达。同一浓度同型半胱氨酸作用72h明显强于24h和48h。结论 同型半胱氨酸可影响血管平滑肌细胞基质金属蛋白酶2的表达，在动脉粥样硬化中可能起着一定的促进作用。Aim To explore the effects of homocysteine （Hey） on the expression of matrix metalloproteinase-2 （MMP- 2） in rat vascular smooth muscle cells （VSMC）, Methods Cultured rat VSMC was incubated with different concentration of Hey in vitro for 24, 18 and 72 h, The expression of MMP-2 was determined by using the methods of gelatin zymography and western blotting. Results 0,05 - 1.0 mmol/L Hey increased the expression of MMP-2 significaatly. Incubated with the same concentration of Hcy the level of MMP-2 of 72 h was higher than that of 24 h and 48 h, Hey 〉 5.0 mmol/L reduced the expression of MMP-2. Incubated with the same concentration of Hey （ 〉 5.0 mmol/L ） the level of MMP-2 of 72 h was lower than that of 24 h and 48 h. Conclusions These datas suggested that Hey can affect the expression of MMP-2 in VSMC. It may be one of factors in the pathogenesis of atherosclerosis induced by Hey,

关 键 词：病理学与病理生理学 同型半胱氨酸致动脉粥样硬化作用 明胶酶谱分析法 同型半胱氨酸 血管平滑肌细胞 基质金属蛋白酶2 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学]