广东人群CYP2C9等位基因及基因型分布频率  

Allele and genotype frequencies of CYP2C9 in Guangdong population

作　　者：何震宇[1] 孙丽敏[2] 李月琴[2] 潘伟[1] 杨红[1] 

机构地区：[1]广东药学院生物化学及分子生物学教研室,广州510006 [2]暨南大学生物工程学系,广州510632

出　　处：《广东医学》2006年第8期1131-1132,共2页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(编号:34051);广东省医学科学技术研究基金资助项目(编号:A2003347)

摘　　要：目的了解广东人群CYP2C9等位基因及基因型分布频率。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对100例健康广东人个体进行CYP2C9基因分型。结果CYP2C91及CYP2C93等位基因频率分别为0.965及0.035,未检测到CYP2C92等位基因;基因型CYP2C91/CYP2C91和CYP2C91/CYP2C93的频率分别为0.930、0.070,符合Hardy-Weinberg平衡分布。结论广东人群CYP2C93等位基因频率与国内其他人群及亚洲其他各国人群相比,差异无显著性,而与欧美高加索人群显著不同。广东人群使用CYP2C9底物药时,应进行相应的CYP2C9基因型检测,以指导临床合理用药和个体化医疗。Objective To investigate the frequencies of the alleles and the genotypes of CYP2Cg in Guangdong population. Methods 103 healthy Guangdong volunteers were studied. The PCR-besed amplification-created restriction site was performed and the PCR products were genotyped by restriction fragment length polymorphism assay. Results The allele frequencies were 0.965 for CYP2C9 ＊ 1 and 0.035 for CYP2C9 ＊ 3 respectively. CYP2C9 ＊ 2 allele was not detected. The frequencies of CYP2C9 ＊ 1/CYP2C9 ＊ 1 and CYP2C9 ＊ 1/CYP2C9 ＊ 3 genotypes were 0.930 and 0.070 respectively,which met Haniy-Wein- berg equilibrium. Condusim No significant difference was observed in the frequencies of the CYP2C9 ＊ 3 allele among Guangdong population and other popnlations in China and other Asian countries. CYP2C9 ＊ 3 frequency in Chinese population was significantly different from those of European and American Caucasians. Genotyping of CYP2C9 will be helpful in guiding rational and individualized medication for individuals from Guangdong population which use drugs metabolized by CYP2C9.

关 键 词：CYP2C9 等位基因 多态性 广东人群 

分 类 号：R394[医药卫生—医学遗传学]