化学预处理对玉米秸秆酶解糖化效果的影响  

Effect of Chemical Pretreatment on Enzymatic Saccharification of Maize Straw

作　　者：宋安东[1,2] 任天宝[3,4] 谢慧[1] 裴广庆[1] 张百良[3] 

机构地区：[1]河南农业大学生命科学学院 [2]农业部农村可再生能源重点开放实验室,河南郑州450002 [3]农业部农村可再生能源重点开放实验室 [4]河南农业大学机电工程学院,河南郑州450002

出　　处：《化学与生物工程》2006年第8期31-33,共3页Chemistry & Bioengineering

基　　金：河南省杰出人才创新基金资助项目(321001300);河南省教育厅自然科学基金项目(2003180061)

摘　　要：研究了化学预处理对玉米秸秆酶解糖化效果的影响。结果表明效果最好的是40目玉米秸秆粉用0·5%的稀盐酸在121℃预处理60min后,加入纤维素酶2.5g·(100g)-1和木聚糖酶液2mL·(100g)-1,在pH4.8的乙酸-乙酸钠缓冲液体系中,液固比为10、50℃下糖化48h,玉米秸秆的总糖产率达48.5%、纤维素和半纤维素的转化率达80.8%。The effect of pretreatment on enzymatic saccharification of maize straw by several chemical substances were studied. The result showed the optimum conditions as follows：the maize straw powder of 40 mesh was pretreated in 0.5% HCl at 121℃ for 60 min,and then was saccarified for 48 h at 50℃ in the buffer solution of acetic acid-sodium acetate of pH 4.8, with the ratio of liquid to solid 10,appending cellulase 2.5 g . （100 g）^-1 and xylanase 2 mL .（100 g）^-1. As a result,the gross saccharification rate reached 48.5% and the transformation rate of cellulose and hemicellulose reached 80.8%.

关 键 词：化学预处理 纤维素酶 木聚糖酶 糖化 

分 类 号：TQ352[化学工程]