Cross-species hybridization of woodchuck hepatitis virus-induced hepatocellular carcinoma using human oligonucleotide microarrays  

作　　者：Paul W Anderson Bud C Tennant Zhenghong Lee 

机构地区：[1]Department of Radiology,University Hospitals of Cleveland, Cleveland, OH 44106,United States [2]Department of Clinical Sciences, College of Veterinary Medicine, Cornell University,Ithaca, NY 14853, United States

出　　处：《World Journal of Gastroenterology》2006年第29期4646-4651,共6页世界胃肠病学杂志（英文版）

基　　金：an NIH grant CA095307 (Z. Lee, PI)by the Gene Expression Array Core Facility of the Comprehensive Cancer Center of Case Western Reserve University and University Hospitals of Cleveland, No. P30 CA43703

摘　　要：AIM： To demonstrate the feasibility of using woodchuck samples on human microarrays, to provide insight into pathways involving positron emission tomography （PET） imaging tracers and to identify genes that could be potential molecular imaging targets for woodchuck hepatocellular carcinoma. METHODS： Labeled cRNA from woodchuck tissue samples were hybridized to Affymetrix U133 plus 2.0 GeneChips. Ten genes were selected for validation using quantitative RT-PCR and literature review was made. RESULTS： Testis enhanced gene transcript （BAX Inhibitor 1）, alpha-fetoprotein, isocitrate dehydrogenase 3 （NAD＋） beta, acetyI-CoA synthetase 2, carnitine palmitoyltransferase 2, and N-myc2 were up-regulated and spermidine/spermine N1-acetyltransferase was down-regulated in the woodchuck HCC. We also found previously published results supporting 8 of the 10 most up-regulated genes and all 10 of the 10 most downregulated genes. CONCLUSION： Many of our microarray results were validated using RT-PCR or literature search. Hence, we believe that woodchuck HCC and non-cancerous liver samples can be used on human microarrays to yield meaningful results.瞄准：为了表明使用土拨鼠的可行性，在人的微数组上取样，到提供卓见进包含正电子排放断层摄影术(宠物) 的小径成像 tracers 并且分子的成像为土拨鼠肝细胞癌指向识别能潜在的基因。方法：从土拨鼠织物样品的标记的 cRNA 是到加 2.0 GeneChips 的 Affymetrix U133 的 hybridized。十基因被选择因为用量的 RT-PCR 和文学评论的确认被做。结果：睾丸提高了基因抄本(BAX 禁止者 1 ) ， alpha-fetoprotein， isocitrate 脱氢酶 3 (NAD+) 贝它， acetyl-CoA 合成酶 2，肉毒碱 palmitoyltransferase 2，并且 N-myc2 是起来调整的，亚精胺 / 精胺 N1-acetyltransferase 在土拨鼠 HCC 是下面调整的。我们也发现了以前出版的结果支持 10 很起来调整的基因中的 8 个和 10 很下面调整的基因中的所有 10 个。结论：许多用 RT-PCR 或文学搜索我们的微数组结果被验证。因此，我们相信土拨鼠 HCC 和非癌的肝样品能在人的微数组上被使用产出有意义的结果。

关 键 词：Cross-species hybridization Gene expression Woodchuck hepatitis virus Hepatocellular carcinoma WOODCHUCK Marmota monax 

分 类 号：R735.7[医药卫生—肿瘤]