桔青霉产美伐他汀发酵条件优化和小罐放大  

Optimization of culture conditions for compactin biosynthesis in Penicillium brevicompactum and further bioprocess development in a bench-top fermentor

作　　者：徐蓉[1] 朱健 吴辉 徐志南[1] 岑沛霖[1] 

机构地区：[1]浙江大学化工与生物工程学系,浙江杭州310027 [2]杭州华东制药股份有限公司技术质量部,浙江杭州310011

出　　处：《化学工程》2006年第8期55-58,共4页Chemical Engineering(China)

摘　　要：首先研究了不同碳、氮源,无机盐,初始pH值和接种量等因素对桔青霉HD-32-2产美伐他汀生产能力的影响,然后着重对4种发酵培养基组分(葡萄糖,麦牙糖,黄豆饼粉和磷酸氢二钾)进行正交试验,形成了最优的发酵培养基。与最初的发酵培养基相比,在最优发酵培养基上发酵水平提高了3倍,达到了986 mg/L,并在15 L小型发酵罐上进行了放大比较研究,进一步说明了优化后培养基具有明显的提高美伐他汀发酵水平的能力。The effects of carbon and nitrogen sources, inorganic salts, initial pH and seed volume on the production of compactin were investigated. One orthogonal experimental procedure was adopted to focus on the optimization of four medium components （glucose, maltose, soybean mill and KEHPO4 ）. The results show that glucose concentration is the most important factor for compactin yield, and the optimal fermentation medium is formulated after this systematic optimization. Compared with the initial fermentation medium, about 3 times improvement in compactin productivity was achieved and the highest productivity attained 986 mg/L after 6 days fermentation. This bioprocess was successfully scaled up by using the formulated medium in a 15 LB.Braun fermentor.

关 键 词：美伐他汀 培养基 发酵条件 桔青霉 

分 类 号：TQ465.1[化学工程—制药化工]