恶性肿瘤患者PCC与DNA含量同步检测的临床意义  

Clinial significance of synchronal measurement of PCC and DNA content in malignant disease.

作　　者：周振英[1] 林琳[1] 戴亮[1] 冯嘉林 邵松生 

机构地区：[1]江苏省肿瘤防治研究所,210009 [2]上海市放射医学研究所

出　　处：《中国优生与遗传杂志》1996年第5期21-23,共3页Chinese Journal of Birth Health & Heredity

摘　　要：作者用熟前凝集染色体(PCC)技术和流式细胞术分别对肿瘤患者外周血细胞G_1-PCC断裂率和癌细胞DNA含量进行了检测.结果表明,肿瘤患者血细胞G_1-PCC断裂率明显升高(P<0.01);患者癌细胞DNA异倍体率为63.2%,其中食管癌为63.0%,宫颈癌为63.3%:S期细胞比率(SPF)明显升高(P<0.01):患者G_1-PCC断裂率随癌细胞DNA指数(DI)增高而增加(r=0.846,r>r_(0.01).异倍体肿瘤患者G_1-PCC断裂率明显高于二倍体患者(P<0.01).随病理组织学分级的增高,G_1-PCC断裂率、癌细胞异倍体检出率和DI值均逐渐升高.因此,对患者血细胞G_1-PCC断裂率和DNA含量同步检测,用于癌患者肿瘤恶性度和预后估计是有一定意义的.GrPCC break rate of peripheral blood cells and DNA content of tumor cells were measured on malignant disease with PCC technique and FCM-DNA analysis as well as the relation between type. The results were as follows. G1-PCC break rate of blood cells are significant increase (P<0. 01). Positive rate of aneuploid cells was 63.2% (containing esophagus cancer 63. 0% and cervical cancer 63. 3%). SPF (S-phase faction) was significal increase in tumor cells (P<0. 01). G1-PCC break rate of blood cells increased with DI (DNA index) of tumor cells (r = 0. 846, r>rO. 01), G1-PCC of blood cells in aneuploid disease was higher than that in diploid disease (P<0. 01). G1-PCC break rate of blood cells, aneuploid rate and DI of tumor cells increased with the raise of histologic grade.

关 键 词：肿瘤 癌 PCC DNA 熟前凝集染色体 染色体畸变 

分 类 号：R730.43[医药卫生—肿瘤]