蛋白激酶Cα反义寡核苷酸对人胰腺癌BXPC-3细胞体外侵袭力的影响  

Effect of protein kinase C-α antisense oligodeoxynucleotides on invasive ability of pancreatic carcinoma BXPC-3 cells in vitro

作　　者：黄涛[1] 冯延平[1] 高军[1] 常青[1] 秦仁义[1] 裘法祖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院外科,武汉430030

出　　处：《郑州大学学报（医学版）》2006年第5期892-894,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:观察蛋白激酶Cα(PKCα)反义寡核苷酸(ASODN)对人胰腺癌BXPC-3细胞体外侵袭力的影响。方法:将BXPC-3细胞分为7组:对照组,64mg/L随机寡核苷酸序列(RODN)转染组,4mg/L、8mg/L、16mg/L、32mg/L和64mg/LASODN转染组,RT-PCR方法检测PKCαmRNA表达情况;以有效转染浓度的ASODN转染BXPC-3细胞,并设对照和RODN组,Transwell侵袭小室方法检测BXPC-3细胞体外侵袭能力。结果:16mg/L、32mg/L和64mg/LASODN转染组PKCα/GADPHmRNA灰度值(0.6371±0.0360,0.5632±0.0080,0.2389±0.0200)均低于对照组(0.9121±0.0250)(P<0.05),RODN组(0.9147±0.0170)、4mg/LASODN组(0.9123±0.0180)和8mg/LASODN组(0.9116±0.0290)与对照组比较差异均无统计学意义(P>0.05)。与对照组穿膜细胞数(286±10)和RODN组(268±3)比较,转染16mg/LPKCαASODN(119±9)可降低胰腺癌BXPC-3细胞的体外侵袭力(P<0.05)。结论:靶向PKCα的ASODN可有效阻断其在人胰腺癌BXPC-3细胞中的表达,并降低癌细胞体外侵袭力。Aim ：To investigate the effect of protein kinase C-α （PKCct） antisense oligodeoxynucleotides （ASODN） on invasive ability of pancreatic carcinoma BXPC-3 cells in vitro. Methods：The cultured BXPC-3 cells were divided into 7 groups： control group, 64 mg/L random oligodeoxynucleotides （RODN） group, 4 mg/L ASODN group, 8 mg/L ASODN group, 16 mg/L ASODN group, 32 mg/L ASODN group, and 64 mg/L ASODN group. The expression of PKCα mRNA was determined by RT-PCR. The invasive ability was measured by the number of cells that penetrated polycarbonates membrane. Results： PKCα/GADPH mRNA relative quantitative ratios of 16 mg/L ASODN group （0. 637 1 ±0. 036 0） , 32 mg/L ASODN group （0. 563 2 ± 0. 008 0） , 64 mg/L ASODN group（0. 238 9±0. 020 0）were significantly lower than that of control group（0. 912 1±0. 0250） （ P 〈0.05 ）. There was no significant difference in the expression of PKCα mRNA among RODN group（0.914 7 ±0.017 0） , 4 mg/L ASODN group（0.912 3 ±0.018 0） , 8 mg/L ASODN group（0.911 6 ±0. 029 0）, and control group （P 〉 0. 05 ）. There were significant differences among 16 mg/L ASODN group （119 ± 9 ）, ROD N group （268 ± 3 ） , and control group （286 ± 10）in the cell number that infiltrated the polycarbonates membrane （P 〈 0.05）. Conclusion ： PKCα ASODN can effectively inhibit the expression of PKCα gene, and decrease the invasion ability of BXPC-3 cells in vitro.

关 键 词：反义寡核苷酸 蛋白激酶CΑ 胰腺肿瘤 癌 BXPC-3细胞 

分 类 号：R730.5[医药卫生—肿瘤]